Antibody detailed product information

Code No.	MT-20-3
Anti-DNA Polymerase α mAb
Price	¥52,800
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 17, 2024 in JST）
Clonality	Monoclonal
Clone	CL22-2-42B
Isotype (Immunized Animal)	Mouse IgG1
Applications	FCM　2 µg/mL (final concentration) IC　2 µg/mL IH　1-2.5 μg/mL　(for cryosection)
Immunogen (Antigen)	purified DNA polymerase α from calf thymus
Reactivity [Gene ID]	Human[5422], Rat(-)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	POLA1
Background	DNA polymerases are essential enzymes involved in DNA replication and in repair. The human genome contains 16 distinct known DNA polymerase genes, and these are classified into four families A, B, X, and Y based on their amino acid sequences. DNA polymerases α (pol α) belong to class B DNA polymerases. The pol α is the only known DNA polymerase able to initiate de novo DNA synthesis and is required for both initiation at chromosomal origins and for the synthesis of Okasaki fragments on the lagging strand. The pol α is composed of four subunits (p180, p68, p58, and p48) with distinctive functions. The polymerase activity is associated with the largest (p180) subunit, whereas the primase activity is associated with the p48 subunit. The p58 subunit contains a nuclear localization signal that is capable of directing both the p58 monomer and the p48-p58 dimer to the nucleus. The p68 subunit binds tightly to the p180 subunit. The p68 subunit is essential for initial DNA synthesis, and is phosphorylated and dephosphorylated in a cell cycle-dependent manner.
Related products	M075-3 Mouse IgG1 (isotype control)
Product category	Research area:Cancer
Data
Citations	Immunohistochemistry Yamaguchi A et al. Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha. Br J Cancer 61,390-3 (1990)(PMID:2328204) Yamaguchi A et al. DNA polymerase alpha positive-cell rate in colorectal cancer and its relationship to prognosis. Br J Cancer 65, 421-4 (1992)(PMID:1558798) Lattanzi G et al. Ultrastructural aspects of the DNA polymerase alpha distribution during the cell cycle. J Histochem Cytochem. 46, 1435-42 (1998)(PMID:9815285)

The availability is based on the information in Japan at 00:05, Apr 17, 2024 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.