Data |
Western Blotting

Western Blotting

Immunoprecipitation

|
Clonality |
Monoclonal
|
Clone |
1E4 |
Isotype
(Immunized Animal)
|
Mouse IgG2aκ
|
Applications |
- WB
- 1-5 µg/mL
 - IP
- 5 µg/sample
- IC
- 5 µg/mL
|
Immunogen
(Antigen) |
17 aa sequence of Auxin Inducible Degron Internal region (mini-AID-tag)
|
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
mini-Degron-tag, mini-Auxin inducible Degron-tag
|
Related products |
PD048 Anti-OsTIR1 pAb
|
Citations |
Western Blotting - Natsume T et al. Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Rep. 15, 210-8 (2016)(PMID:27052166)
- Schuller AP et al. eIF5A Functions Globally in Translation Elongation and Termination. Mol Cell. 66, 194-205.e5 (2017)(PMID:28392174)
- Natsume T et al. Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. 31, 816-829 (2017)(PMID:28487407)
- Shen EZ et al. Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline. Cell.172, 937-951.e18 (2018)(PMID:29456082)
- Davidson L et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep. 26, 2779-2791.e5 (2019)(PMID:30840897)
- Yesbolatova A et al. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 11, 5701 (2020)
(PMID:33177522)
|
References |
- Nishimura K and Kanemaki MT et al. Rapid depletion of budding yeast proteins via the fusion of an Auxin-Inducible Degron (AID). Curr Protoc Cell Biol. 64, 20.9.1-20.9.16 (2014)(PMID: 25181302)
- Kubota T et al. The Elg1 replication factor C-like complex fuctions in PCNA unloading during DNA replication. Mol Cell. 50, 273-80 (2013)(PMID: 23499004)
- Nishimura K et al. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6, 917-22 (2009)(PMID: 19915560)
|
Product category |
- Tools
- Epitope tags
|