Antibody detailed product information

Code No. M214-3
Anti-mini-AID-tag mAb
Price ¥55,500
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 20, 2026 in JST）
Clonality Monoclonal
Clone 1E4
Isotype
(Immunized Animal) 		Mouse IgG2aκ
Applications WB　1-5 µg/mL  
IP　5 µg/sample  
IC　5 µg/mL  
Immunogen
(Antigen)		 17 aa sequence of Auxin Inducible Degron Internal region (mini-AID-tag)
Storage buffer "1 mg/mL in PBS/50% glycerol, pH 7.2"
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names "mini-Degron-tag, mini-Auxin inducible Degron-tag"
Related products PD048 Anti-OsTIR1 pAb
Product category Tools:Epitope tags
Data



Citations

Western Blotting

  1. Natsume T et al. Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Rep. 15, 210-8 (2016)(PMID:27052166)
  2. Schuller AP et al. eIF5A Functions Globally in Translation Elongation and Termination. Mol Cell. 66, 194-205.e5 (2017)(PMID:28392174)
  3. Natsume T et al. Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. 31, 816-829 (2017)(PMID:28487407)
  4. Shen EZ et al. Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline. Cell.172, 937-951.e18 (2018)(PMID:29456082)
  5. Davidson L et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep. 26, 2779-2791.e5 (2019)(PMID:30840897)
  6. Yesbolatova A et al. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 11, 5701 (2020) (PMID:33177522)
References
  1. Nishimura K and Kanemaki MT et al. Rapid depletion of budding yeast proteins via the fusion of an Auxin-Inducible Degron (AID). Curr Protoc Cell Biol. 64, 20.9.1-20.9.16 (2014)（PMID: 25181302）
  2. Kubota T et al. The Elg1 replication factor C-like complex fuctions in PCNA unloading during DNA replication. Mol Cell. 50, 273-80 (2013)（PMID: 23499004）
  3. Nishimura K et al. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6, 917-22 (2009)（PMID: 19915560）
  • The availability is based on the information in Japan at 00:05, Apr 20, 2026 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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