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HOME > Product search results > Code No. D291-7 Anti-His-tag mAb-HRP-DirecT

Code No. D291-7

Anti-His-tag mAb-HRP-DirecT

Price

¥22,000

Availability (in Japan)

10 or more

(In Japan at 17:00,
Oct 20, 2019 in JST)

Size

50 µL

Data
  • Western Blotting

Clonality Monoclonal Clone OGHis
Isotype (Immunized Animal) Mouse IgG2aκ
Applications
WB
1:5,000  
Immunogen (Antigen) 6xHis tagged protein
Storage buffer PBS/Preservative/Stabilizer
Storage temp. 4°C Conjugate HRP Manufacturer MBL
Background The His-tag (6xHis-tag) is one of the most common tags used to facilitate the purification of recombinant proteins. Metal chelate affinity chromatography is widely used for purification of His-tagged proteins. This specific antibody is useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of the recombinant proteins.
Related products PM032 Anti-His-tag pAb
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D291-3S Anti-His-tag mAb
D291-6 Anti-His-tag mAb-Biotin
D291-8 Anti-His-tag mAb-Agarose
D291-10 Anti-His-tag mAb-Magnetic Agarose
D291-A48 Anti-His-tag mAb-Alexa Fluor® 488
D291-A59 Anti-His-tag mAb-Alexa Fluor® 594
D291-A64 Anti-His-tag mAb-Alexa Fluor® 647
M089-3 Anti-His-tag mAb
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Citations
  1. Lin Q et al. The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways. Nat Commun. 6, 7981 (2015)(PMID:26272249)

Western Blotting

  1. Yamaguchi A et al. In vitro characterization of the RS motif in N-terminal head domain of goldfish germinal vesicle lamin B3 necessary for phosphorylation of the p34cdc2 target serine by SRPK1. FEBS Open Bio. 3, 165-76 (2013)(PMID:23772390)
  2. Sakata K et al. HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. Sci Rep. 3, 3243 (2013).(PMID:24263861)
  3. Maki M et al. Transglutaminase-catalyzed incorporation of polyamines masks the DNA-binding region of the transcription factor Relish. J Biol Chem. 292, 6369-6380 (2017)(PMID:28258224)
  4. Shibata T et al. Drosophila TG-A transglutaminase is secreted via an unconventional Golgi-independent mechanism involving exosomes and two types of fatty acylations. J Biol Chem. 292, 10723-10734 (2017)(PMID:28476891)
  5. Takeya T et al. A Pichia pastoris single-cell biosensor for detection of enzymatically produced methanol. Appl Microbiol Biotechnol. 102, 7017-7027 (2018)(PMID:29948115)
  6. Nakamura M et al. Functional characterization of unique enzymes in Xanthomonas euvesicatoria related to degradation of arabinofurano-oligosaccharides on hydroxyproline-rich glycoproteins. PLoS One 13, e0201982 (2018)(PMID:30092047)
  7. Nakashima K et al. Chitin-based barrier immunity and its loss predated mucus-colonization by indigenous gut microbiota. Nat Commun. 9, 3402 (2018) (PMID:30143642)
  8. Umehara T et al. Lysine Acetylation Regulates Alanyl-tRNA Synthetase Activity in Escherichia coli. Genes 9, E473 (2018)(PMID:30274179)
Product category
Tools
Epitope tags
Brands
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  • The availability is based on the information in Japan at 17:00, Oct 20, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.