This site is for customers in Japan. Customers in other regions, please go to Global page.
HOME > Product search results > Code No. PM032 Anti-His-tag pAb

Code No. PM032

Anti-His-tag pAb

Price

¥40,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Oct 23, 2019 in JST)

Size

100 µL

Data
  • Western Blotting

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
5 µL/sample  
IC*
reported.  (PMID: 21216843
Immunogen (Antigen) Carrier protein conjugated 6xHissynthetic peptide
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Expression vectors containing a protein and a tag peptide are commonly used. His-tag fusion protein expression system is preferably used in various laboratories, because its simple protein purification step by heavy metal, such as nickel, affinity chromatography. This specific antibody for His-tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification.
Related products PM032-8 Anti-His-tag pAb-Agarose
D291-3 Anti-His-tag mAb
D291-3S Anti-His-tag mAb
D291-6 Anti-His-tag mAb-Biotin
D291-7 Anti-His-tag mAb-HRP-DirecT
D291-8 Anti-His-tag mAb-Agarose
D291-10 Anti-His-tag mAb-Magnetic Agarose
D291-A48 Anti-His-tag mAb-Alexa Fluor® 488
D291-A59 Anti-His-tag mAb-Alexa Fluor® 594
D291-A64 Anti-His-tag mAb-Alexa Fluor® 647
M089-3 Anti-His-tag mAb
M136-3 Anti-His-tag mAb
D291-11 Anti-His-tag mAb-Magnetic Beads
Citations

Western Blotting

  1. Shibata Y et al. Activation of the IκEB kinase complex by HTLV-1 Tax requires cytosolic factors involved in Tax-induced polyubiquitination. J Biochem. 150, 679-86 (2011)(PMID:21862596)
  2. Kajikawa M et al. The intertransmembrane region of Kaposi's sarcoma-associated herpesvirus modulator of immune recognition 2 contributes to B7-2 downregulation. J Virol. 86, 5288-96 (2012)(PMID:22379101)
  3. Nishiyama A et al. Uhrf1-dependent H3K23 ubiquitylation couples maintenance DNA methylation and replication. Nature. 502, 249-53 (2013)(PMID:24013172)
  4. Lee Y et al. Structure of the triose-phosphate/phosphate translocator reveals the basis of substrate specificity. Nat Plants. 3, 825-832 (2017)(PMID:28970497)
  5. Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res. 28, 756-770 (2018)(PMID:29844578)
  6. Fukui R et al. Cleavage of Toll-Like Receptor 9 Ectodomain Is Required for In Vivo Responses to Single Strand DNA. Front Immunol. 9, 1491 (2018)(PMID:29997629)
  7. Kang J et al. FIH permits NAA10 to catalyze the oxygen-dependent lysyl-acetylation of HIF-1α. Redox Biol. 19, 364-374 (2018)(PMID:30237125)
  8. Li M et al. p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis. Cell Death Dis. 9, 941 (2018)(PMID:30237540)
  9. Gao T et al. PKCζ Phosphorylates SIRT6 to Mediate Fatty Acid β-Oxidation in Colon Cancer Cells. Neoplasia. 21, 61-73 (2019)(PMID:30504065)

Immunoprecipitation

  1. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  2. Zhang L et al. POH1 deubiquitinates pro-interleukin-1β and restricts inflammasome activity. Nat Commun. 9, 4225 (2018)(PMID:30315153)

Immunocytochemistry

  1. Takemoto M et al. Laminar and areal expression of unc5d and its role in cortical cell survival. Cereb Cortex. 21, 1925-34 (2011)(PMID:21216843)
Product category
Tools
Epitope tags
Brands
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Oct 23, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.