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HOME > Product search results > Code No. D153-8 Anti-GFP (Green Fluorescent Protein) mAb-Agarose

Code No. D153-8

Anti-GFP (Green Fluorescent Protein) mAb-Agarose

Price

¥60,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Nov 22, 2019 in JST)

Size

Gel: 200 µL

Data
  • Immunoprecipitation

Clonality Monoclonal Clone RQ2
Isotype (Immunized Animal) Rat IgG2aκ
Applications
IP
20 µL of gel slurry  
ChIP*
reported.  (PMID: 16618814 / 23616927
Co-IP*
reported.  (PMID: 22006334 / 22027834 / 22167059
Other(Purification)
reported.  (PMID: 23204472
Other(CLIP)
reported.  (PMID: 29125873
Immunogen (Antigen) GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP
Storage buffer 100 µg of anti-GFP monoclonal antibody covalently coupled to 200 µL of agarose gel and provided as a 50% gel slurry suspended in PBS containing preservative (0.1% ProClin 150)
Storage temp. 4°C Conjugate Agarose Manufacturer MBL
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D153-6 Anti-GFP mAb-Biotin
Citations

ChIP

  1. Darzacq X et al. Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells. J Cell Biol. 173, 207-18 (2006)(PMID:16618814)
  2. Celesnik H et al. Arabidopsis thaliana VOZ (Vascular plant One-Zinc finger) transcription factors are required for proper regulation of flowering time. Biol Open. 2,  424-31 (2013)(PMID:23616927)
  3. Yu X et al. The chromatin remodeler ZmCHB101 impacts expression of osmotic stress-responsive genes in maize. Plant Mol Biol. 97, 451-465 (2018)(PMID:29956114)

Co-IP

  1. Sasabe M et al. Phosphorylation of a mitotic kinesin-like protein and a MAPKKK by cyclin-dependent kinases (CDKs) is involved in the transition to cytokinesis in plants. PNAS 108,17844-9 (2011)(PMID:22006334)
  2. Fukumoto Y et al. Role of scaffold protein afadin dilute domain-interacting protein (ADIP) in platelet-derived growth factor-induced cell movement by activating Rac protein through Vav2 protein. J Biol Chem. 286, 43537-48 (2011)(PMID:22027834)
  3. Aung K, Hu J. The Arabidopsis tail-anchored protein PEROXISOMAL AND MITOCHONDRIAL DIVISION FACTOR1 is involved in the morphogenesis and proliferation of peroxisomes and mitochondria. Plant Cell 23, 4446-61 (2011)(PMID:22147290)
  4. Heyman J et al. Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome. Plant Cell 23, 4394-410 (2011)(PMID:22167059)
  5. Liu Q et al. Complementation of Hyponastic Leaves1 by Double-strand RNA Binding Domains of Dicer-like 1 in Nuclear Dicing Bodies. Plant Physiol. 163, 108-17 (2013)(PMID:23886622)
  6. Liu J et al. MicroRNA319-regulated TCPs interact with FBHs and PFT1 to activate CO transcription and control flowering time in Arabidopsis. PLoS Genet. 13, e1006833 (2017)(PMID:28558040)
  7. Dong J et al. Light-Dependent Degradation of PIF3 by SCFEBF1/2 Promotes a Photomorphogenic Response in Arabidopsis. Curr Biol. 27, 2420-2430.e6 (2017)(PMID:28736168)
  8. Song X et al. IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice. Cell Res. 27, 1128-1141 (2017)(PMID:28809396)
  9. Wang S et al. MKK4 from Litopenaeus vannamei is a regulator of p38 MAPK kinase and involved in anti-bacterial response. Dev Comp Immunol. 78, 61-70 (2018)(PMID:28939483)
  10. Yu X et al. The chromatin remodeler ZmCHB101 impacts expression of osmotic stress-responsive genes in maize. Plant Mol Biol. 97, 451-465 (2018)(PMID:29956114)

Immunoprecipitation

  1. Hayakawa T et al. ACT domain repeat protein 7, ACR7, interacts with a chaperone HSP18.0-CII in rice nuclei. Plant Cell Physiol. 47, 891-904 (2006)(PMID:16720649)
  2. Tanaka T et al. Drosophila Mon2 couples Oskar-induced endocytosis with actin remodeling for cortical anchorage of the germ plasm. Development 138, 2523-32 (2011)(PMID:21610029)
  3. Shirae-Kurabayashi M et al. Ci-Pem-1 localizes to the nucleus and represses somatic gene transcription in the germline of Ciona intestinalis embryos. Development 138, 2871-81 (2011)(PMID:21693510)
  4. Zhong Y et al. Importin beta interacts with the endoplasmic reticulum-associated degradation machinery and promotes ubiquitination and degradation of mutant alpha1-antitrypsin. J Biol Chem. 286, 33921-30 (2011)(PMID:21832065)
  5. Cai L et al. Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genes. J Biol Chem. 286, 35915-21 (2011)(PMID:21880729)
  6. Roberts D et al. Modulation of phototropic responsiveness in Arabidopsis through ubiquitination of phototropin 1 by the CUL3-Ring E3 ubiquitin ligase CRL3(NPH3). Plant Cell 23, 3627-40 (2011)(PMID:21990941)
  7. Henderson MJ et al. Mesencephalic astrocyte-derived neurotrophic factor (MANF) secretion and cell surface binding are modulated by KDEL receptors. J Biol Chem. 288, 4209-25 (2013)(PMID:23255601)
  8. Paster W et al. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J Immunol.190,  3749-56 (2013)(PMID:23460737)
  9. He M et al. Systematic Analysis of the Functions of Lysine Acetylation in the Regulation of Tat Activity. PLoS One 8, e67186 (2013)(PMID:23826228)
  10. Choi HI et al. Peroxiredoxin V selectively regulates IL-6 production by modulating the Jak2-Stat5 pathway. Free Radic Biol Med. 65, 270-279 (2013)(PMID:23831231)
  11. Tomida J et al. REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ. Nucleic Acids Res. 43, 1000-11 (2015)(PMID:25567983)
  12. Underhill SM et al. Differential regulation of two isoforms of the glial glutamate transporter EAAT2 by DLG1 and CaMKII. J Neurosci. 35, 5260-70 (2015)(PMID:25834051)
  13. Okatsu K et al. Phosphorylated ubiquitin chain is the genuine Parkin receptor. J Cell Biol. 209, 111-28 (2015)(PMID:25847540)
  14. Hodder AN et al. Structural basis for plasmepsin V inhibition that blocks export of malaria proteins to human erythrocytes. Nat Struct Mol Biol. 22, 590-6 (2015)(PMID:26214367)
  15. Wang D et al. Splicing Factor Prp8 Interacts With NES(AR) and Regulates Androgen Receptor in Prostate Cancer Cells. Mol Endocrinol. 29, 1731-42 (2015)(PMID:26371515)
  16. Tatavosian R et al. Distinct Cellular Assembly Stoichiometry of Polycomb Complexes on Chromatin Revealed by Single-molecule Chromatin Immunoprecipitation Imaging. J Biol Chem. 290, 28038-54 (2015)(PMID:26381410)
  17. Boddey JA et al. Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding. Nat Commun. 7, 10470 (2016)(PMID:26832821)
  18. Nadarajan S et al. The MAP kinase pathway coordinates crossover designation with disassembly of synaptonemal complex proteins during meiosis. Elife. 5, e12039 (2016)(PMID:26920220)
  19. Brown KC et al. ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline. Nucleic Acids Res. 45, 9093-9107 (2017)(PMID:28645154)
  20. Niida H et al. Phosphorylated HBO1 at UV irradiated sites is essential for nucleotide excision repair. Nat Commun. 8, 16102 (2017)(PMID:28719581)
  21. Lüningschrör P et al. Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease. Nat Commun. 8, 678 (2017)(PMID:29084947)
  22. Kageyama S et al. Negative regulation of the Keap1-Nrf2 pathway by a p62/Sqstm1 splicing variant. Mol Cell Biol. (2018) In press.(PMID:29339380)
  23. Tomida J et al. FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells. EMBO J. 37, e99543 (2018)(PMID:29789392)
  24. Xu Z et al. O-GlcNAcylation regulates integrin-mediated cell adhesion and migration via formation of focal adhesion complexes. J Biol Chem. 294, 3117-3124 (2019)(PMID:30587575)

Other(Purification)

  1. Yen YT et al. Differential localization of the streptococcal accessory sec components and implications for substrate export. J Bacteriol. 195, 682-95 (2013)(PMID:23204472)

Other(CLIP)

  1. Yahara M et al. U6 snRNA expression prevents toxicity in TDP-43-knockdown cells. PLoS One. 12, e0187813 (2017)(PMID:29125873)
Product category
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Epitope tags
Fluorescent proteins
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  • The availability is based on the information in Japan at 00:05, Nov 22, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.