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HOME > Product search results > Code No. M048-3 Anti-GFP (Green Fluorescent Protein) mAb

Code No. M048-3

Anti-GFP (Green Fluorescent Protein) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Nov 19, 2019 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

  • Immunohistochemistry

  • Immunohistochemistry

Clonality Monoclonal Clone 1E4
Isotype (Immunized Animal) Mouse IgG2b
Applications
WB
1 µg/mL  
IP
5 µg/sample  
IC
2 µg/mL  
IH
10 µg/mL  
IF*
reported.  (PMID: 26581759
Immunogen (Antigen) Recombinant full-length GFP (246 a.a.)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry.
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Citations

Western Blotting

  1. Abe Y et al. Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog. FEBS J. 275, 318-31 (2008)(PMID:18081866)
  2. Komuro A et al. Diffuse-type gastric carcinoma: progression, angiogenesis, and transforming growth factor beta signaling. J Natl Cancer Inst. 101, 592-604 (2009)(PMID:19351925)
  3. Takahata M et al. Ro52 functionally interacts with IgG1 and regulates its quality control via the ERAD system. Mol Immunol. 45, 2045-54 (2008)(PMID:18022694)
  4. Masuda M et al. The tumor suppressor protein TSLC1 is involved in cell-cell adhesion. J Biol Chem. 277, 31014-9 (2002)(PMID:12050160)
  5. Nakamichi I et al. Formation of Mallory body-like inclusions and cell death induced by deregulated expression of keratin 18. Mol Biol Cell 13, 3441-51 (2002)(PMID:12388748)
  6. Song Z et al. Direct interaction between survivin and Smac/DIABLO is essential for the anti-apoptotic activity of survivin during taxol-induced apoptosis. J Biol Chem. 278, 23130-40 (2003)(PMID:12660240)
  7. de Graaf K et al. Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain: phosphorylation by DYRK1A and colocalization with splicing factors. J Biol Chem. 279, 4612-24 (2004)(PMID:14623875)
  8. Iwai S et al. A novel actin-bundling kinesin-related protein from Dictyostelium discoideum. J Biol Chem. 279, 4696-704 (2004)(PMID:14623897)
  9. Lozupone F et al. Identification and relevance of the CD95-binding domain in the N-terminal region of ezrin. J Biol Chem. 279, 9199-207 (2004)(PMID:14676203)
  10. Pottekat A, Menon AK. Subcellular localization and targeting of N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the second enzyme in the glycosylphosphatidylinositol biosynthetic pathway. J Biol Chem. 279, 15743-51 (2004)(PMID:14742432)
  11. Hisatsune C et al. Regulation of TRPC6 channel activity by tyrosine phosphorylation. J Biol Chem. 279, 18887-94 (2004)(PMID:14761972)
  12. Kontrogianni-Konstantopoulos A et al. Obscurin regulates the organization of myosin into A bands. Am J Physiol Cell Physiol. 287, C209-17 (2004)(PMID:15013951)
  13. Hanamoto T et al. Identification of protein kinase A catalytic subunit beta as a novel binding partner of p73 and regulation of p73 function. J Biol Chem. 280, 16665-75 (2005)(PMID:15723830)
  14. Reversi A et al. Effects of cholesterol manipulation on the signaling of the human oxytocin receptor. Am J Physiol Regul Integr Comp Physiol. 291, R861-9 (2006)(PMID:16966388)
  15. Takeda K et al. Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1. J Biol Chem. 282, 7522-31 (2007)(PMID:17210579)
  16. Lebel-Haziv Y et al. Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs. Neoplasia 16, 723-40 (2014)(PMID:25246273)
  17. Zhang B et al. GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis. PLoS Biol. 13, e1002129 (2015)(PMID:25860027)
  18. Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res. 28, 756-770 (2018)(PMID:29844578)
  19. Tsukune N et al. Forced expression of mouse progerin attenuates the osteoblast differentiation interrupting β-catenin signal pathway in vitro. Cell Tissue Res. 375, 655-664 (2019)(PMID:30284086)

Immunoprecipitation

  1. Banerjee S et al. A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation. Neuron. 64, 871-84 (2009)(PMID:20064393)
  2. Asada N, Sanada K. LKB1-mediated spatial control of GSK3beta and adenomatous polyposis coli contributes to centrosomal forward movement and neuronal migration in the developing neocortex. J Neurosci. 30, 8852-65 (2010)(PMID:20592207)
  3. Kiriyama M et al. Interaction of FLASH with arsenite resistance protein 2 is involved in cell cycle progression at S phase. Mol Cell Biol. 29, 4729-41 (2009)(PMID:19546234)
  4. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells 14, 1155-65 (2009)(PMID:19735482)
  5. Federici C et al. Pleiotropic function of ezrin in human metastatic melanomas. Int J Cancer. 124, 2804-12 (2009)(PMID:19235924)
  6. Ozoe F et al. The 14-3-3 proteins Rad24 and Rad25 negatively regulate Byr2 by affecting its localization in Schizosaccharomyces pombe. Mol Cell Biol. 22, 7105-19 (2002)(PMID:12242289)
  7. de Graaf K et al. Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain: phosphorylation by DYRK1A and colocalization with splicing factors. J Biol Chem. 279, 4612-24 (2004)(PMID:14623875)
  8. Lozupone F et al. Identification and relevance of the CD95-binding domain in the N-terminal region of ezrin. J Biol Chem. 279, 9199-207 (2004)(PMID:14676203)
  9. Lebel-Haziv Y et al. Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs. Neoplasia 16, 723-40 (2014)(PMID:25246273)
  10. Zhang B et al. GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis. PLoS Biol. 13, e1002129 (2015)(PMID:25860027)
  11. Watanabe N et al. Dystonia-4 (DYT4)-associated TUBB4A mutants exhibit disorganized microtubule networks and inhibit neuronal process growth. Biochem Biophys Res Commun. 495, 346-352 (2018)(PMID:29127012)
  12. Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res. 28, 756-770 (2018)(PMID:29844578)

Immunocytochemistry

  1. Banerjee S et al. A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation. Neuron. 64, 871-84 (2009)(PMID:20064393)
  2. Asano Y et al. DRR1 is expressed in the developing nervous system and downregulated during neuroblastoma carcinogenesis. Biochem Biophys Res Commun. 394, 829-35 (2010)(PMID:20298674)
  3. Ko HS et al. Role of ubiquilin associated with protein-disulfide isomerase in the endoplasmic reticulum in stress-induced apoptotic cell death. J Biol Chem. 277, 35386-92 (2002)(PMID:12095988)

Immunohistochemistry

  1. Broday L et al. The LIM domain protein UNC-95 is required for the assembly of muscle attachment structures and is regulated by the RING finger protein RNF-5 in C. elegans. J Cell Biol. 165, 857-67 (2004)(PMID:15210732)
  2. Sugawara T et al. Type 1 inositol trisphosphate receptor regulates cerebellar circuits by maintaining the spine morphology of purkinje cells in adult mice. J Neurosci. 33, 12186-96 (2013)(PMID:23884927)
  3. Katsushima K et al. Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment. Nat Commun. 7, 13616 (2016)(PMID:27922002)
  4. Shainer I et al. Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish. Sci Rep. 7, 44777 (2017)(PMID:28317906)

Immunofluorescence

  1. Sun J et al. Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation. Nat Commun. 6, 8856 (2015)(PMID:26581759)
  2. Shimizu T et al. The C. elegans BRCA2-ALP/Enigma Complex Regulates Axon Regeneration via a Rho GTPase-ROCK-MLC Phosphorylation Pathway. Cell Rep. 24, 1880-1889 (2018)(PMID:30110643)
Product category
Tools
Epitope tags
Fluorescent proteins
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  • The availability is based on the information in Japan at 00:05, Nov 19, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.