Clonality |
Monoclonal
|
Clone |
3H9 |
Isotype
(Immunized Animal)
|
Mouse IgG1
|
Applications |
- FCM
- 20 µL (Ready for use.)
|
Immunogen
(Antigen) |
PMA activated human neutrophil
|
Reactivity
[Gene ID] |
Human[8875], Mouse(-), Rat(-) |
Storage buffer |
PBS/1% BSA/0.09% NaN3
|
Storage temp. |
冷蔵
|
Conjugate |
PE |
Manufacturer |
MBL |
Alternative names |
VNN2, vanin 2, FOAP-4, GPI-80
|
Background |
The GPI-80 molecule (80 kDa) recognized by this antibody (clone 3H9) was shown to be present on human neutrophils. When 3H9 was added with a neutrophil stimulant (fMLP), the inhibition of neutrophil adherence was observed after 60 minutes incubation. 3H9 enhanced not only fMLP-induced chemotaxis but random migration of neutrophils as well. Furthermore, 3H9 clearly discriminated neutrophils from both basophils and eosinophils derived from humans. |
Related products |
D087-3 Anti-GPI-80 (Human) mAb
|
Citations |
Flow Cytometry - Saeki K et al. A feeder-free and efficient production of functional neutrophils from human embryonic stem cells. Stem Cells. 27, 59-67 (2009)(PMID:18845766)
- Dou DR et al. Medial HOXA genes demarcate haematopoietic stem cell fate during human development. Nat Cell Biol. 18, 595-606 (2016)(PMID:27183470)
- Takeda Y et al. The pattern of GPI-80 expression is a useful marker for unusual myeloid maturation in peripheral blood. Clin Exp Immunol. 186, 373-386 (2016)(PMID:27569996)
- Ng ES et al. Differentiation of human embryonic stem cells to HOXA+ hemogenic vasculature that resembles the aorta-gonad-mesonephros. Nat Biotechnol. 34, 1168-1179 (2016)(PMID:27748754)
- Sumide K et al. A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells. Nat Commun. 9, 2202 (2018)(PMID:29875383)
- Takeda Y et al. An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes Using Flow Cytometry. Methods Mol Biol. 1916, 177-193 (2019)(PMID:30535695)
- Kato T et al. GPI-80 as a Useful Index for Myeloid Cell Heterogeneity and a Potential Prognostic Biomarker for Metastatic Renal Cell Carcinoma. Tohoku J Exp Med. 249, 203-212 (2019)(PMID:31776298)
- Bornhauser B et al. The hematopoietic stem cell marker VNN2 is associated with chemoresistance in pediatric B-cell precursor ALL. Blood Adv. 4, 4052-4064 (2020)(PMID:32853382)
|
Product category |
- Research area
- Cell surface antigens
|