Antibody detailed product information

Code No. D087-5
Anti-GPI-80 (Human) mAb-PE
Price ¥44,000
Size 1 mL (50 tests)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 24, 2024 in JST）
Clonality Monoclonal
Clone 3H9
Isotype
(Immunized Animal) 		Mouse IgG1
Applications FCM　20 µL (Ready for use.)  
Immunogen
(Antigen)		 PMA activated human neutrophil
Reactivity [Gene ID]

Human[8875], Mouse(-), Rat(-)
Storage buffer PBS/1% BSA/0.09% NaN3
Storage temp. 4°C
Conjugate PE
Manufacturer MBL
Alternative names VNN2, vanin 2, FOAP-4, GPI-80
Background The GPI-80 molecule (80 kDa) recognized by this antibody (clone 3H9) was shown to be present on human neutrophils. When 3H9 was added with a neutrophil stimulant (fMLP), the inhibition of neutrophil adherence was observed after 60 minutes incubation. 3H9 enhanced not only fMLP-induced chemotaxis but random migration of neutrophils as well. Furthermore, 3H9 clearly discriminated neutrophils from both basophils and eosinophils derived from humans.
Related products D087-3 Anti-GPI-80 (Human) mAb
Product category Research area:Cell surface antigens
Citations

Flow Cytometry

  1. Saeki K et al. A feeder-free and efficient production of functional neutrophils from human embryonic stem cells. Stem Cells. 27, 59-67 (2009)(PMID:18845766)
  2. Dou DR et al. Medial HOXA genes demarcate haematopoietic stem cell fate during human development. Nat Cell Biol. 18, 595-606 (2016)(PMID:27183470)
  3. Takeda Y et al. The pattern of GPI-80 expression is a useful marker for unusual myeloid maturation in peripheral blood. Clin Exp Immunol. 186, 373-386 (2016)(PMID:27569996)
  4. Ng ES et al. Differentiation of human embryonic stem cells to HOXA+ hemogenic vasculature that resembles the aorta-gonad-mesonephros. Nat Biotechnol. 34, 1168-1179 (2016)(PMID:27748754)
  5. Sumide K et al. A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells. Nat Commun. 9, 2202 (2018)(PMID:29875383)
  6. Takeda Y et al. An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes Using Flow Cytometry. Methods Mol Biol. 1916, 177-193 (2019)(PMID:30535695)
  7. Kato T et al. GPI-80 as a Useful Index for Myeloid Cell Heterogeneity and a Potential Prognostic Biomarker for Metastatic Renal Cell Carcinoma. Tohoku J Exp Med. 249, 203-212 (2019)(PMID:31776298)
  8. Bornhauser B et al. The hematopoietic stem cell marker VNN2 is associated with chemoresistance in pediatric B-cell precursor ALL. Blood Adv. 4, 4052-4064 (2020)(PMID:32853382)
  • The availability is based on the information in Japan at 17:00, Apr 24, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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