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HOME > Product search results > Code No. D086-3 Anti-ASC (TMS1) (Human) mAb

Code No. D086-3

Anti-ASC (TMS1) (Human) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 17:00,
Jan 18, 2020 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

  • Immunoprecipitation

Clonality Monoclonal Clone 23-4
Isotype (Immunized Animal) Mouse IgG1
Applications
WB
1 μg/mL  
IP
5 μg/300 μL of cell extract from 5x106 HL-60 cells  
IC*
reported.  (PMID: 10567338 / 15041718
IH*
reported.  (PMID: 11561011
Immunogen (Antigen) Triton X-100 insoluble fraction of retinoic acid-treated HL-60 cells
Reactivity [Gene ID]

Human[29108], Mouse(-), Rat(-)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names PYCARD, TMS, CARD5, TMS-1
Background ASC (apoptosis-associated speck-like protein containing a CARD (caspase recruitment domain)) is a 22 kDa soluble protein, located in the cytosol of HL-60 cells. In apoptotic HL-60 cells, it is able to be visualized as a speck, forming an insoluble aggregate. The C-terminal domain of this protein contains a CARD, suggesting that ASC may have proapoptotic activity in HL-60 cells. Recent data have indicated that ASC plays a role of adaptor protein linking various PAAD (Pyrin, AIM, ASC, and death domain-like) family proteins to pathways involved in NF-κB and procaspase-1 activation.
Related products D086-A64 Anti-ASC (TMS1) (Human) mAb-Alexa Fluor® 647
Citations
  1. Masumoto J et al. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 274, 33835-8 (1999)(PMID:10567338)
  1. Masumoto J et al. Murine ortholog of ASC, a CARD-containing protein, self-associates and exhibits restricted distribution in developing mouse embryos. Exp Cell Res. 262, 128-33 (2001)(PMID:11139337)
  1. Masumoto J et al. Pyrin N-terminal homology domain- and caspase recruitment domain-dependent oligomerization of ASC. Biochem Biophys Res Commun. 280, 652-5 (2001)(PMID:11162571)

Western Blotting

  1. Mayor A et al. A crucial function of SGT1 and HSP90 in inflammasome activity links mammalian and plant innate immune responses. Nat Immunol. 8, 497-503. (2007)(PMID:17435760)
  2. Bryan NB et al. Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J Inflamm (Lond). 7, 23 (2010)(PMID:20482797)

Immunocytochemistry

  1. Bryan NB et al. Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J Inflamm (Lond). 7, 23 (2010)(PMID:20482797)
  2. Terasawa K et al. Epigenetic inactivation of TMS1/ASC in ovarian cancer. Clin Cancer Res. 10, 2000-6 (2004)(PMID:15041718)

Immunohistochemistry

  1. Masumoto J et al. Expression of apoptosis-associated speck-like protein containing a caspase recruitment domain, a pyrin N-terminal homology domain-containing protein, in normal human tissues. J Histochem Cytochem. 49, 1269-75 (2001)(PMID:11561011)
Product category
Research area
Immunology
Apoptosis
Brands
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  • The availability is based on the information in Japan at 17:00, Jan 18, 2020 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.