Data |
Western Blotting
Immunoprecipitation
|
Clonality |
Monoclonal
|
Clone |
23-4 |
Isotype
(Immunized Animal)
|
Mouse IgG1
|
Applications |
- WB
- 1 µg/mL
- IP
- 5 µg/300 µL of cell extract from 5x106 HL-60 cells
- IC*
- reported. (PMID: 10567338 / 15041718)
- IH*
- reported. (PMID: 11561011)
|
Immunogen
(Antigen) |
Triton X-100 insoluble fraction of retinoic acid-treated HL-60 cells
|
Reactivity
[Gene ID] |
Human[29108], Mouse(-), Rat(-) |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
PYCARD, TMS, CARD5, TMS-1
|
Background |
ASC (apoptosis-associated speck-like protein containing a CARD (caspase recruitment domain)) is a 22 kDa soluble protein, located in the cytosol of HL-60 cells. In apoptotic HL-60 cells, it is able to be visualized as a speck, forming an insoluble aggregate. The C-terminal domain of this protein contains a CARD, suggesting that ASC may have proapoptotic activity in HL-60 cells. Recent data have indicated that ASC plays a role of adaptor protein linking various PAAD (Pyrin, AIM, ASC, and death domain-like) family proteins to pathways involved in NF-κB and procaspase-1 activation. |
Related products |
D086-A64 Anti-ASC (TMS1) (Human) mAb-Alexa Fluor™ 647
|
Citations |
- Masumoto J et al. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 274, 33835-8 (1999)(PMID:10567338)
- Masumoto J et al. Murine ortholog of ASC, a CARD-containing protein, self-associates and exhibits restricted distribution in developing mouse embryos. Exp Cell Res. 262, 128-33 (2001)(PMID:11139337)
- Masumoto J et al. Pyrin N-terminal homology domain- and caspase recruitment domain-dependent oligomerization of ASC. Biochem Biophys Res Commun. 280, 652-5 (2001)(PMID:11162571)
Western Blotting - Mayor A et al. A crucial function of SGT1 and HSP90 in inflammasome activity links mammalian and plant innate immune responses. Nat Immunol. 8, 497-503. (2007)(PMID:17435760)
- Bryan NB et al. Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J Inflamm (Lond). 7, 23 (2010)(PMID:20482797)
Immunocytochemistry - Bryan NB et al. Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J Inflamm (Lond). 7, 23 (2010)(PMID:20482797)
- Terasawa K et al. Epigenetic inactivation of TMS1/ASC in ovarian cancer. Clin Cancer Res. 10, 2000-6 (2004)(PMID:15041718)
Immunohistochemistry - Masumoto J et al. Expression of apoptosis-associated speck-like protein containing a caspase recruitment domain, a pyrin N-terminal homology domain-containing protein, in normal human tissues. J Histochem Cytochem. 49, 1269-75 (2001)(PMID:11561011)
|
Product category |
- Research area
- Immunology
Apoptosis
|