Data |
Western Blotting

Immunoprecipitation

RNP Immunoprecipitation

|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit Ig (aff.)
|
Applications |
- WB
- 1:1,000
 - IP
- 5 µg/250 µL of cell extract from 2.5 x 106 cells
- IC
- Can be used.
- RIP
- 15 µg/500 µL of cell extract from 1.5 x 107 cells

|
Immunogen
(Antigen) |
KLH-conjugated synthetic peptide MSNGYEDHMAEDCRGDIGR (1-19 aa)
|
Reactivity
[Gene ID] |
Human[1994](PMID: 23176121), Mouse[15568], Rat[363854], Hamster[100754131] |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R), HUR, Hua, MelG, ELAV1/HUR, W91709, 2410055N02Rik
|
Background |
ELAVL1/HuR, like other ELAVL proteins, has a short N-terminal region followed by 2 RNA-binding motifs, a basic linker domain, and a third RNA-binding motif. The basic hinge region bears a novel nuclear shuttling sequence which is implicated in the nucleocytoplasmic HuR shuttling. Unlike its neuron-specific ELAV relatives (HuB, HuC and HuD), HuR itself is ubiquitously expressed. Structure-function analyses of HuR have shown that it binds to AU-rich mRNA sequences in the nucleus and may be involved in their nuclear export. |
Related products |
RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN005P Anti-ELAVL2 (HuB) (Human) pAb RN006P Anti-ELAVL3 (HuC) pAb RN007M Anti-ELAVL1 (HuR) mAb
|
Citations |
Western Blotting - Onomoto K et al. Critical role of an antiviral stress granule containing RIG-I and PKR in viral detection and innate immunity. PLoS One. 7, e43031 (2012)(PMID:22912779)
RNP Immunoprecipitation - Williams TK et al. pp32 (ANP32A) expression inhibits pancreatic cancer cell growth and induces gemcitabine resistance by disrupting HuR binding to mRNAs. PLoS One. 5, e15455 (2010)(PMID:21152064)
- Pineda DM et al. HuR's post-transcriptional regulation of Death Receptor 5 in pancreatic cancer cells. Cancer Biol Ther. 13, 946-55 (2012)(PMID:22785201)
- Banadakoppa M et al. Role of transcription factor Sp1 and RNA binding protein HuR in the downregulation of Dr(+) Escherichia coli receptor protein decay accelerating factor (DAF or CD55) by nitric oxide. FEBS J. 280, 840-54 (2013)(PMID:23176121)
- Jinbo M et al. Targeting the mRNA-binding protein HuR impairs malignant characteristics of pancreatic ductal adenocarcinoma cells. Oncotarget 6, 27312-31 (2015)(PMID:26314962)
- Suhl JA et al. A 3' untranslated region variant in FMR1 eliminates neuronal activity-dependent translation of FMRP by disrupting binding of the RNA-binding protein HuR. PNAS 112, E6553-61 (2015)(PMID:26554012)
- Huang YH et al. Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy. Oncotarget 7, 21812-24 (2016)(PMID:26943573)
- Romeo C et al. HuR contributes to TRAIL resistance by restricting Death Receptor 4 expression in pancreatic cancer cells. Mol Cancer Res. 14, 599-611 (2016)(PMID:27053682)
- Popovitchenko T et al. The RNA binding protein HuR determines the differential translation of autism-associated FoxP subfamily members in the developing neocortex. Sci. Rep. 6, 28998 (2016)(PMID:27383233)
- Zhou H et al. GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR. Cell Death Dis. 7, e2294 (2016)(PMID:27415424)
|
Product category |
- Research area
- RNA-RNP Network
Cancer - Brands
- RiboCluster Profiler™
|