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HOME > Product search results > Code No. D031-3H Anti-Proton Pump (H,K-ATPase α subunit) mAb

Code No. D031-3H

Anti-Proton Pump (H,K-ATPase α subunit) mAb



Availability (in Japan)

10 or more

(In Japan at 17:00,
Sep 23, 2020 in JST)


6 mL

  • Immunohistochemistry

Clonality Monoclonal Clone 1H9
Isotype (Immunized Animal) Mouse IgG1
Ready for use  
Reactivity [Gene ID]

Human[495], Mouse[11944], Rat[24216], Bovine[521163]

Storage temp. 4°C Conjugate Unlabeled Manufacturer MBL
Alternative names ATP4A, ATPase H+/K+ transporting alpha subunit
Background Hydrogen-potassium adenosine triphosphatase (H+, K+-ATPase) belongs to a family of P-type cation-transporting ATPases that also includes Ca2+-ATPase and Na+, K+-ATPase. H+, K+-ATPase is responsible for acid secretion into the stomach and catalyzes electroneutral exchange of cytoplasmic hydrogen ions and external potassium ions coupled with ATP hydrolysis. The enzyme is found in gastric parietal cells where it is located in cytoplasmic vesicles or apical plasma membranes of the secretory canaliculus. H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic a subunit and a smaller but heavily glycosylated b subunit. The a subunit of H+, K+-ATPase has 10 transmembrane domains and contains sites for ATP binding and its acylphosphorylation, binding sites of proton pump inhibitors, and sites responsible for ion recognition.
Related products D031-3 Anti-Proton Pump (H,K-ATPase α subunit) mAb
D032-3H Anti-Proton Pump (H,K-ATPase β subunit) mAb
D032-3 Anti-Proton Pump (H,K-ATPase β subunit) mAb
Notes This hybridoma (clone 1H9) was established by fusion of mouse myeloma cell Sp2/0-Ag14 with BALB/c nu/+ Mouse splenocyte with neonatal thymectomy-induced autoimmune gastritis.

Western Blotting

  1. Fujii T et al. Non-morphogenic effect of Sonic Hedgehog on gastric H+,K+-ATPase activity. Biochem Biophys Res Commun. 518, 605-609 (2019)(PMID:31445709)


  1. Ghanem LR et al. Specific enrichment of the RNA-binding proteins PCBP1 and PCBP2 in chief cells of the murine gastric mucosa. Gene Expr Patterns. 14, 78-87 (2014)(PMID:24480778)
  2. Ogawa T et al. CHAC1 overexpression in human gastric parietal cells with Helicobacter pylori infection in the secretory canaliculi. Helicobacter. 24, e12598 (2019)(PMID:31111570)
  3. Fujii T et al. Non-morphogenic effect of Sonic Hedgehog on gastric H+,K+-ATPase activity. Biochem Biophys Res Commun. 518, 605-609 (2019)(PMID:31445709)
Product category
Molecular pathology tools

  • The availability is based on the information in Japan at 17:00, Sep 23, 2020 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.