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HOME > Product search results > Code No. PD026 Anti-Atg14 pAb

Code No. PD026

Anti-Atg14 pAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Jul 30, 2021 in JST)

Size

100 µL

Data
  • Western Blotting

  • Immunoprecipitation

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:500  
IP
5 µL/300 µL of cell extract from 3 x 106 cells  
IC*
reported.  (PMID: 24705551
Immunogen (Antigen) Recombinant Human Atg14 (167-404 a.a.)
Reactivity [Gene ID]

Human[22863], Mouse[100504663], Rat[305831], Hamster(-)

Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names ATG14, autophagy related 14, ATG14L, BARKOR, KIAA0831/ Atg14L, 4832427M01, D14Ertd114e, D14Ertd436e
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Mammalian homologues of Atg14/Atg14L/BARKOR localizes on the isolation membrane and autophagosome, and it necessary for autophagosome formation. Atg14 binds to the PI3K complex (Beclin-1, Vps34, Vps15) and promotes the autophagosome formation.
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PM072 Anti-VMP1 pAb
Citations

Western Blotting

  1. Hamasaki M et al. Autophagosomes form at ER-mitochondria contact sites. Nature 495, 389-93 (2013)(PMID:23455425)
  2. Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421)
  3. Kim C, Bergelson JM. Echovirus 7 entry into polarized caco-2 intestinal epithelial cells involves core components of the autophagy machinery. J Virol. 88, 434-43 (2014)(PMID:24155402)
  4. Bejarano E et al. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 16, 401-14 (2014)(PMID:24705551)

Immunoprecipitation

  1. Kim J et al. Differential regulation of distinct Vps34 complexes by AMPK in nutrient stress and autophagy. Cell 152, 290-303 (2013)(PMID:23332761)
  2. Zhong Y et al. Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. J Biol Chem. 289, 26021-37 (2014)(PMID:25086043)
  3. Nemazanyy I et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 6, 8283 (2015)(PMID:26387534)

Immunocytochemistry

  1. Bejarano E et al. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 16, 401-14 (2014)(PMID:24705551)
  2. Shizukuishi S et al. Streptococcus pneumoniae hijacks host autophagy by deploying CbpC as a decoy for Atg14 depletion. EMBO Rep. 21, e49232 (2020)(PMID:32239622)
Product category
Research area
Autophagy
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  • The availability is based on the information in Japan at 00:05, Jul 30, 2021 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.