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HOME > Product search results > Code No. MI-12-1 Anti-EGF-R (Human) mAb

Code No. MI-12-1

Anti-EGF-R (Human) mAb

Price

¥32,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Dec 16, 2019 in JST)

Size

100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Flow Cytometry

  • Immunocytochemistry

Clonality Monoclonal Clone 6F1
Isotype (Immunized Animal) Mouse IgG2bκ
Applications
WB
1:1,000 - 1:2,000  
IP
2-5 µL/250 µL of cell extract from 2.5x106 cells  
FCM
1:100 (final concentration)  
IC
1:100  
Immunogen (Antigen) Recombinant human EGF-R intracellular region (648-1186 a.a.)
Reactivity [Gene ID]

Human[1956]

Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Related products M220-3 Anti-HB-EGF mAb
D308-3 Anti-HB-EGF (Human) mAb
Citations
  1. Lazarovici P et al. Down-regulation of epidermal growth factor receptors by nerve growth factor in PC12 cells is p140(trk)-, Ras-, and Src-dependent. J Biol Chem. 272, 11026-34 (1997)(PMID:9110995)
  1. Ishikawa K et al. EGFR-dependent phosphorylation of leucine-rich repeat kinase LRRK1 is important for proper endosomal trafficking of EGFR. Mol Biol Cell. 23, 1294-306 (2012)(PMID:22337768)

Western Blotting

  1. Ichiba T et al. Enhancement of guanine-nucleotide exchange activity of C3G for Rap1 by the expression of Crk, CrkL, and Grb2. J Biol Chem. 272, 22215-20 (1997)(PMID:9268367)
  2. Shibutani M et al. Transcriptional down-regulation of epidermal growth factor receptors by nerve growth factor treatment pf PC12 cells. J Biol Chem. 273, 6878-84 (1998)(PMID:9506991)
  3. Lazarovici P et al. The 38-amino-acid form of pituitary adenylate cyclase-activating polypeptide induces neurite outgrowth in PC12 cells that is dependent on protein kinase C and extracellular signal-regulated kinase but not on protein kinase A, nerve growth factor receptor tyrosine kinase, p21(ras) G protein, and pp60(c-src) cytoplasmic tyrosine kinase. Mol Pharmacol. 54, 547-58 (1998)(PMID:9730914)
  4. Liu XW at al. The Wilms' tumor gene product WT1 mediates the down-regulation of the rat epidermal growth factor receptor by nerve growth factor in PC12 cells. J Biol Chem. 276, 5068-73 (2001)(PMID:11071895)
  5. Mizuno E et al. Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes. Mol Biol Cell. 16, 5163-74 (2005)(PMID:16120644)
  6. Yamasaki A et al. The Ca2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A. Mol Biol Cell. 17, 4876-87 (2006)(PMID:16957052)
  7. Chung I et al. Spatial control of EGF receptor activation by reversible dimerization on living cells. Nature 464, 783-7 (2010)(PMID:20208517)
  8. Spiess C et al. Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies. Nat Biotechnol. 31, 753-8 (2013)(PMID:23831709)
  9. Hasegawa J et al. Autophagosome-lysosome fusion in neurons requires INPP5E, a protein associated with Joubert syndrome. EMBO J. 35, 1853-67 (2016)(PMID:27340123)
  10. Kondo H et al. Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms. Hum Mol Genet. 26, 173-183 (2017)(PMID:28013294)
  11. Hori I et al. Defects in autophagosome-lysosome fusion underlie Vici syndrome, a neurodevelopmental disorder with multisystem involvement. Sci Rep. 7, 3552 (2017)(PMID:28615637)

Immunoprecipitation

  1. Hashimoto Y et al. Phosphorylation of CrkII adaptor protein at tyrosine 221 by epidermal growth factor receptor. J Biol Chem. 273, 17186-91 (1998)(PMID:9642287)
  2. Mizuno E et al. Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes. Mol Biol Cell. 16, 5163-74 (2005)(PMID:16120644)

Flow Cytometry

  1. Muta Y et al. Composite regulation of ERK activity dynamics underlying tumour-specific traits in the intestine. Nat Commun. 9, 2174 (2018)(PMID:29872037)

Immunocytochemistry

  1. Liu XW at al. The Wilms' tumor gene product WT1 mediates the down-regulation of the rat epidermal growth factor receptor by nerve growth factor in PC12 cells. J Biol Chem. 276, 5068-73 (2001)(PMID:11071895)
  2. Nakamura T et al. Grit, a GTPase-activating protein for the Rho family, regulates neurite extension through association with the TrkA receptor and N-Shc and CrkL/Crk adapter molecules. Mol Cell Biol. 22, 8721-34 (2002)(PMID:12446789)
  3. Mizuno E et al. STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif. Mol Biol Cell. 14, 3675-89 (2003)(PMID:12972556)
  4. Mizuno E et al. Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes. Mol Biol Cell. 16, 5163-74 (2005)(PMID:16120644)

Immunohistochemistry

  1. Hara T et al. Copper(II) Bis(diethyldithiocarbamate) Induces the Expression of Syndecan-4, a Transmembrane Heparan Sulfate Proteoglycan, via p38 MAPK Activation in Vascular Endothelial Cells. Int J Mol Sci. 19, 3302 (2018)(PMID:30352976)
Product category
Research area
Cell surface antigens
Cytokine & Growth Factors
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Dec 16, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.