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HOME > Product search results > Code No. M185-3L Anti-DDDDK-tag mAb

Code No. M185-3L

Anti-DDDDK-tag mAb

Price

¥51,700

Availability (in Japan)

10 or more

(In Japan at 17:00,
Apr 18, 2024 in JST)

Size

1mL (1 mg/mL)

Data
  • Western Blotting

  • Immunoprecipitation

  • Flow Cytometry

  • Immunocytochemistry

Clonality Monoclonal Clone FLA-1
Isotype (Immunized Animal) Mouse IgG2aκ
Applications
WB
0.1 µg/mL  
IP
2 µg/sample  
FCM
0.05 µg/mL  
IC
0.1 µg/mL  
ChIP*
reported.  (PMID: 28634046
Co-IP*
reported.  (PMID: 26871431
Other(Immuno-TEM)
reported.  (PMID: 32771428
Immunogen (Antigen) KLH-conjugated synthetic peptide, DYKDDDDK
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Related products M185-7 Anti-DDDDK-tag mAb-HRP-DirecT
M185-A48 Anti-DDDDK-tag mAb-Alexa Fluor™ 488
M185-A59 Anti-DDDDK-tag mAb-Alexa Fluor™ 594
M185-A64 Anti-DDDDK-tag mAb-Alexa Fluor™ 647
PM020 Anti-DDDDK-tag pAb
PM020-7 Anti-DDDDK-tag pAb-HRP-DirecT
PM020-8 Anti-DDDDK-tag pAb-Agarose
M185-6 Anti-DDDDK-tag mAb-Biotin
Citations
  1. Watanabe S et al. MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites. Sci Rep. 8, 10888 (2018)(PMID:30022091)

Western Blotting

  1. Konogami T et al. Ligand-dependent responses of the silkworm prothoracicotropic hormone receptor, Torso, are maintained by unusual intermolecular disulfide bridges in the transmembrane region. Sci Rep. 6, 222437 (2016)(PMID:26928300)
  2. Hossain MS et al. Neuronal Orphan G-Protein Coupled Receptor Proteins Mediate Plasmalogens-Induced Activation of ERK and Akt Signaling. PLoS One. 11, e0150846 (2016)(PMID:26934370)
  3. Fan L et al. FAM122A, a new endogenous inhibitor of protein phosphatase 2A. Oncotarget 7, 63887-63900 (2016)(PMID:27588481)
  4. Feng S et al. Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to Promote Restart of Stalled Replication Forks. J Biol Chem. 291, 21956-21962 (2016)(PMID:27601467)
  5. Makino S et al. Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci Rep. 6, 39608 (2016)(PMID:28000783)
  6. Song KH et al. REP1 inhibits FOXO3-mediated apoptosis to promote cancer cell survival. Cell Death Dis. 8, e2536 (2017)(PMID:28055019)
  7. Lee YK et al. Tumor antigen PRAME is up-regulated by MZF1 in  cooperation with DNA hypomethylation in melanoma cells. Cancer Lett. 403, 144-151 (2017)(PMID:28634046)
  8. Yang J et al. Isocitrate protects DJ-1 null dopaminergic cells from oxidative stress through NADP+-dependent isocitrate dehydrogenase (IDH). PLoS Genet. 13, e1006975 (2017)(PMID:28827794)
  9. Zhou Y et al. Cellular RNA Helicase DDX1 Is Involved in Transmissible Gastroenteritis Virus nsp14-Induced Interferon-Beta Production. Front Immunol. 8, 940 (2017)(PMID:28848548)
  10. Wang S et al. Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis. Cell Death Dis. 8, e3058 (2017)(PMID:28933784)
  11. Huang Y et al. RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21. Oncotarget. 8, 83075-83087 (2017)(PMID:29137325)
  12. Ma XX et al. PiT2 regulates neuronal outgrowth through interaction with microtubule-associated protein 1B. Sci Rep. 7, 17850 (2017)(PMID:29259219)
  13. Nishida KM et al. Hierarchical roles of mitochondrial Papi and Zucchini in Bombyx germline in piRNA biogenesis. Nature 555, 260-264 (2018)(PMID:29489748)
  14. Deng T et al. Deubiquitylation and stabilization of p21 by USP11 is critical for cell-cycle progression and DNA damage responses. PNAS. 115, 4678-4683 (2018)(PMID:29666278)
  15. Tu R et al. USP49 participates in the DNA damage response by forming a positive feedback loop with p53. Cell Death Dis. 9, 553 (2018)(PMID:29748582)
  16. Nahorski MS et al. Biallelic UFM1 and UFC1 mutations expand the essential role of ufmylation in brain development. Brain 141, 1934-1945 (2018)(PMID:29868776)
  17. Yang L et al. Reconstituting Arabidopsis CRY2 Signaling Pathway in Mammalian Cells Reveals Regulation of Transcription by Direct Binding of CRY2 to DNA. Cell Rep. 24, 585-593.e4(2018)(PMID:30021157)
  18. Xu SH et al. ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell Death Dis. 9, 479 (2018)(PMID:29706618)
  19. Matsumoto S et al. Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting. Nucleic Acids Res. 46, 8079-8089 (2018)(PMID:30085309)
  20. Song X et al. Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells. BMC Cancer. 18, 971 (2018)(PMID:30309319)
  21. Du DF et al. Updated Genetic Testing of Primary Hyperoxaluria Type 1 in a Chinese Population: Results from a Single Center Study and a Systematic Review. Curr Med Sci. 38, 749-757 (2018)(PMID:30341509)
  22. Haq S et al. The stability and oncogenic function of LIN28A are regulated by USP28. Biochim Biophys Acta Mol Basis Dis. 1865, 599-610 (2019)(PMID:30543854)
  23. Adachi N et al. Differential S-palmitoylation of the human and rodent β3-adrenergic receptors. J Biol Chem. 294, 2569-2578 (2019)(PMID:30541923)
  24. Zhang L et al. Overexpression of MTA1 inhibits the metastatic ability of ZR-75-30 cells in vitro by promoting MTA2 degradation. Cell Commun Signal. 17, 4 (2019) (PMID:30642362)
  25. Wang Y et al. Cancer/testis Antigen MAGEA3 Interacts with STAT1 and Remodels the Tumor Microenvironment. Int J Med Sci. 15, 1702-1712 (2018)(PMID:30588194)
  26. Chen M et al. TBC1D8 Amplification Drives Tumorigenesis through Metabolism Reprogramming in Ovarian Cancer. Theranostics. 9, 676-690 (2019)(PMID:30809301)
  27. Liang L et al. Deubiquitylase USP7 regulates human terminal erythroid differentiation by stabilizing GATA1. Haematologica. in press.(PMID:30872372)
  28. Lin Let al. An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS). PLoS Pathog. 15, e1007795 (2019)(PMID:31170267)
  29. Cui X et al. Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro. Viruses. 11, pii: E559 (2019)(PMID:31212927)
  30. Masai H et al. Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities. Sci Rep. 9, 8618 (2019)(PMID:31197198)
  31. Moon S et al. Asxl1 exerts an antiproliferative effect on mouse lung maturation via epigenetic repression of the E2f1-Nmyc axis. Cell Death Dis. 9 1118 (2018)(PMID:30389914)
  32. Hong CS et al. Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1. Front Immunol. 10, 1760 (2019)(PMID:31402917)
  33. Xu G et al. Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex. PLoS Pathog. 15, e1008002 (2019)(PMID:31404116)
  34. Chen XH et al. Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis. Acta Pharmacol Sin. 40, 1568-1577 (2019)(PMID:31197245)
  35. Koyano F et al. Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes. EMBO Rep. 20, e47728 (2019)(PMID:31602805)
  36. Huang Y et al. Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance. Cells. 8, pii: E1264 (2019)(PMID:31623230)
  37. Yang CC et al. Cdc7 Activates Replication Checkpoint by Phosphorylating the Chk1-binding Domain of Claspin in Human Cells. Elife. 8, e50796 (2019) (PMID:31889509)
  38. Song KH et al. HSP90A Inhibition Promotes Anti-Tumor Immunity by Reversing Multi-Modal Resistance and Stem-Like Property of Immune-Refractory Tumors. Nat Commun. 11, 562 (2020)(PMID:31992715)
  39. Zhu S et al. An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis. Nat Commun. 11, 1685 (2020)(PMID:32245947)
  40. Liu S et al. Porcine deltacoronavirus (PDCoV) infection antagonizes interferon-λ1 production. Vet Microbiol. 247, 108785 (2020)(PMID:32768229)
  41. Kato T et al. Preparation of divalent antigen-displaying enveloped virus-like particles using a single recombinant Bombyx mori nucleopolyhedrovirus bacmid in silkworms. J Biotechnol. 323, 92-97 (2020)(PMID:32771428)
  42. Gao X et al. The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells. Nat Commun. 11, 3904 (2020)(PMID:32764536)
  43. Jang YG et al. Mislocalization of TORC1 to Lysosomes Caused by KIF11 Inhibition Leads to Aberrant TORC1 Activity. Mol Cells. 43, 705-717 (2020)(PMID:32759469)
  44. Xu Y et al. Anti-bacterial and anti-viral nanchangmycin displays anti-myeloma activity by targeting Otub1 and c-Maf. Cell Death Dis. 11, 818 (2020) (PMID:32999280)
  45. He T et al. Duck enteritis virus pUL47, as a late structural protein localized in the nucleus, mainly depends on residues 40 to 50 and 768 to 777 and inhibits IFN-β signalling by interacting with STAT1. Vet Res. 51, 135 (2020) (PMID:33176874)
  46. Kim YJ et al. O-GlcNAc stabilizes SMAD4 by inhibiting GSK-3β-mediated proteasomal degradation. Sci Rep. 10, 19908 (2020) (PMID:33199824)
  47. Yamaoka Y et al. Zika virus protease induces caspase-independent pyroptotic cell death by directly cleaving gasdermin D. Biochem Biophys Res Commun. 534, 666-671 (2021) (PMID:33208231)

ChIP

  1. Lee YK et al. Tumor antigen PRAME is up-regulated by MZF1 in  cooperation with DNA hypomethylation in melanoma cells. Cancer Lett. 403, 144-151 (2017)(PMID:28634046)
  2. Song KH et al. HSP90A Inhibition Promotes Anti-Tumor Immunity by Reversing Multi-Modal Resistance and Stem-Like Property of Immune-Refractory Tumors. Nat Commun. 11, 562 (2020)(PMID:31992715)

Co-IP

  1. Wu T et al. Expression of Ferritin Light Chain (FTL) Is Elevated in Glioblastoma, and FTL Silencing Inhibits Glioblastoma Cell Proliferation via the GADD45/JNK Pathway. PLoS One. 11, e0149361 (2016)(PMID:26871431)
  2. Xu SH et al. ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell Death Dis. 9, 479 (2018)(PMID:29706618)
  3. Wang Y et al. Cancer/testis Antigen MAGEA3 Interacts with STAT1 and Remodels the Tumor Microenvironment. Int J Med Sci. 15, 1702-1712 (2018)(PMID:30588194)
  4. Moon S et al. Asxl1 exerts an antiproliferative effect on mouse lung maturation via epigenetic repression of the E2f1-Nmyc axis. Cell Death Dis. 9 1118 (2018)(PMID:30389914)
  5. Huang J et al. SR-BI Interactome Analysis Reveals a Proviral Role for UGGT1 in Hepatitis C Virus Entry. Front Microbiol. 10, 3389 (2019)(PMID:31551978)
  6. Zhao Z et al. Functional analysis of rare variants of GATA4 identified in Chinese patients with congenital heart defect. Genesis. 57, e23333 (2019)(PMID:31513339)
  7. Huang Y et al. Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance. Cells. 8, pii: E1264 (2019)(PMID:31623230)

Immunoprecipitation

  1. Konogami T et al. Ligand-dependent responses of the silkworm prothoracicotropic hormone receptor, Torso, are maintained by unusual intermolecular disulfide bridges in the transmembrane region. Sci Rep. 6, 222437 (2016)(PMID:26928300)
  2. Song KH et al. REP1 inhibits FOXO3-mediated apoptosis to promote cancer cell survival. Cell Death Dis. 8, e2536 (2017)(PMID:28055019)
  3. Xu Y et al. 53BP1 and BRCA1 control pathway choice for stalled replication restart. Elife. 6, e30523 (2017)(PMID:29106372)
  4. Huang Y et al. RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21. Oncotarget. 8, 83075-83087 (2017)(PMID:29137325)
  5. Ma XX et al. PiT2 regulates neuronal outgrowth through interaction with microtubule-associated protein 1B. Sci Rep. 7, 17850 (2017)(PMID:29259219)
  6. Deng T et al. Deubiquitylation and stabilization of p21 by USP11 is critical for cell-cycle progression and DNA damage responses. PNAS. 115, 4678-4683 (2018)(PMID:29666278)
  7. Tu R et al. USP49 participates in the DNA damage response by forming a positive feedback loop with p53. Cell Death Dis. 9, 553 (2018)(PMID:29748582)
  8. Haq S et al. The stability and oncogenic function of LIN28A are regulated by USP28. Biochim Biophys Acta Mol Basis Dis. 1865, 599-610 (2019)(PMID:30543854)
  9. Chen M et al. TBC1D8 Amplification Drives Tumorigenesis through Metabolism Reprogramming in Ovarian Cancer. Theranostics. 9, 676-690 (2019)(PMID:30809301)
  10. Hong CS et al. Gal-3BP Negatively Regulates NF-κB Signaling by Inhibiting the Activation of TAK1. Front Immunol. 10, 1760 (2019)(PMID:31402917)
  11. Jang YG et al. Mislocalization of TORC1 to Lysosomes Caused by KIF11 Inhibition Leads to Aberrant TORC1 Activity. Mol Cells. 43, 705-717 (2020) (PMID:32759469)

Immunocytochemistry

  1. Feng S et al. Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to Promote Restart of Stalled Replication Forks. J Biol Chem. 291, 21956-21962 (2016)(PMID:27601467)
  2. Mitsui E et al. Identification of ryuvidine as a KDM5A inhibitor. Sci Rep. 9, 9952 (2019)(PMID:31289306)

Immunohistochemistry

  1. Koyano F et al. Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes. EMBO Rep. 20, e47728 (2019)(PMID:31602805)

ELISA

  1. Shimizu Y et al. Anti-tumor effect of a recombinant Bifidobacterium strain secreting a claudin-targeting molecule in a mouse breast cancer model. Eur J Pharmacol. 887, 173596 (2020) (PMID:32979353)

RNP Immunoprecipitation

  1. Yamaguchi S et al. Crystal structure of Drosophila Piwi. Nat Commun. 11, 858 (2020)(PMID:32051406)

Other(Immuno-TEM)

  1. Kato T et al. Preparation of divalent antigen-displaying enveloped virus-like particles using a single recombinant Bombyx mori nucleopolyhedrovirus bacmid in silkworms. J Biotechnol. 323, 92-97 (2020)(PMID:32771428)
Product category
Tools
Epitope tags
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  • The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.