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HOME > Product search results > Code No. M148-3M Anti-monomeric Kusabira-Green N-terminal fragment mAb

Code No. M148-3M

Anti-monomeric Kusabira-Green N-terminal fragment mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 17:00,
Dec 11, 2019 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

Clonality Monoclonal Clone 1E6
Isotype (Immunized Animal) Mouse IgG2b
Applications
WB
1 µg/mL  
IC*
  (PMID: 22911572
Immunogen (Antigen) mKG_N (1-168 a.a.)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names mKG_N
Background CoralHue® Fluo-chase Kit can detect protein-protein interactions as fluorescent signals using the protein fragment complementation method. The gene of CoralHue® monomeric Kusabira-Green (mKG), a reporter protein, is divided into two fragments (CoralHue® mKG_N fragment and CoralHue® mKG_C fragment) which are respectively fused to the target protein genes to investigate the interactions. When the expressed target proteins don’t interact, CoralHue® mKG_N fragment and CoralHue® mKG_C fragment cannot approach each other and can not emit fluorescence. However, when target proteins interact, divided CoralHue® mKG fragments spatially approach each other and the local effective concentration increases. As a result, mKG fragments form a steric structure before dividing and the chromophore emits fluorescence. The fluorescent signals can be detected depending on the fused target protein-protein interactions.
Related products M149-3M Anti-monomeric Kusabira-Green C-terminal fragment mAb
M168-3M Anti-monomeric Kusabira-Orange 2 mAb
Notes This antibody (clone 1E6) has the epitope on CoralHue® mKG_N fragment and can detect the fusion protein with CoralHue® mKG_N specifically.
Citations

Immunocytochemistry

  1. Nitta S et al. Hepatitis C virus NS4B protein targets STING and abrogates RIG-I-mediated type I interferon-dependent innate immunity. Hepatology 57, 46-58 (2013)(PMID:22911572)
Product category
Tools
Fluorescent proteins
Brands
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  • The availability is based on the information in Japan at 17:00, Dec 11, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.