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HOME > Product search results > Code No. M044-3 Anti-XIAP (MIHA/ILP-a) mAb

Code No. M044-3

Anti-XIAP (MIHA/ILP-a) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 17:00,
Aug 6, 2020 in JST)

Size

100 µg/100 µL

This antibody recognizes C-terminal region of XIAP (352-449 aa) and detects 55 kDa of XIAP on Western Blot using total cell lysate from human or mouse cell line, for example, Jurkat, Raji, NIH/3T3, etc.
Data
  • Western Blotting

Clonality Monoclonal Clone 2F1
Isotype (Immunized Animal) Mouse IgG1
Applications
WB
1 µg/mL  
IH
reported.  (PMID: 15576477 / 18056177 / 11489828
Immunogen (Antigen) Recombinant Human full length XIAP
Reactivity [Gene ID]

Human[331], Mouse[11798], Rat(w)[63879]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names X-linked inhibitor of apoptosis, API3, ILP1, XLP2, BIRC4, IAP-3, hIAP3, hIAP-3
Background Caspase, related to ICE and to CED-3, plays central roles as effectors of apoptosis. Ablation of caspase activity is attained by p35 from baculovirus and CrmA from cowpox, which appear to be suicide inactivators, strongly inhibiting caspase activity. Overexpression of there caspase inhibitors in insect, nematoda and mammalian cells results in resistance to apoptotic stimuli, demonstrating that components of the apoptotic pathway are highly conserved throughout evolution, and leading to the speculation that mammalian functional equivalents of these protease inhibitors may exist. The inhibitor of apoptosis proteins (IAPs) are a family of anti-apoptotic proteins that are conserved across species. Four IAPs have been identified in mammal; NAIP, cIAP1/HIAP2/hMIHB, cIAP2/HIAP1/hMIHC, XIAP/hILP. A prototype of the human IAPs is the XIAP, with a 1.5 kb coding region corresponding to a 55 kDa protein. XIAP can directly inhibit two members of the cell death protease family, caspase-3 and -7.
Related products M097-3 Anti-Caspase-3 (Human) mAb
M087-3 Anti-Caspase-3 (Human) mAb
M053-3 Anti-Caspase-7 mAb
M054-3 Anti-Caspase-9 mAb
Citations

Western Blotting

  1. Silke J, et al., Unlike Diablo/smac, Grim promotes global ubiquitination and specific degradation of X chromosome-linked inhibitor of apoptosis (XIAP) and neither cause apoptosis. J Biol Chem. 279, 4313-21. 2004(PMID:14570909)
  2. Tanaka H et al. 3-Phosphoinositide-dependent protein kinase-1-mediated IkappaB kinase beta (IkkB) phosphorylation activates NF-kappaB signaling. J Biol Chem. 280, 40965-73 (2005)(PMID:16207722)
  3. Silke J et al. Determination of cell survival by RING-mediated regulation of inhibitor of apoptosis (IAP) protein abundance. PNAS. 102, 16182-7 (2005)(PMID:16263936)
  4. Marsden VS et al. Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death. Blood. 107, 1872-7 (2006)(PMID:16291596)
  5. Cillessen SA et al. Inhibition of the intrinsic apoptosis pathway downstream of caspase-9 activation causes chemotherapy resistance in diffuse large B-cell lymphoma. Clin Cancer Res. 1, 7012-21 (2007)(PMID:18056177)
  6. Smith MI et al. Skeletal muscle differentiation evokes endogenous XIAP to restrict the apoptotic pathway. PLoS One 4, e5097 (2009)(PMID:19333375)
  7. Verzola D et al. Uric acid promotes apoptosis in human proximal tubule cells by oxidative stress and the activation of NADPH oxidase NOX 4. PLoS One 9, e115210 (2014)(PMID:25514209)
  8. Ishikawa C et al. Mitotic kinase PBK/TOPK as a therapeutic target for adult T‑cell leukemia/lymphoma. Int J Oncol. 53, 801-814 (2018)(PMID:29901068)
  9. Vince JE et al. The Mitochondrial Apoptotic Effectors BAX/BAK Activate Caspase-3 and -7 to Trigger NLRP3 Inflammasome and Caspase-8 Driven IL-1β Activation. Cell Rep. 25, 2339-2353.e4 (2018) (PMID:30485804)
  10. Ishikawa C et al. Evaluation of Artesunate for the Treatment of Adult T-cell leukemia/lymphoma. Eur J Pharmacol. 872, 172953 (2020)(PMID:31996318)

Immunohistochemistry

  1. Ferreira CG et al. Assessment of IAP (inhibitor of apoptosis) proteins as predictors of response to chemotherapy in advanced non-small-cell lung cancer patients. Ann Oncol. 12, 799-805 (2001)(PMID:11484955)
  2. Ferreira CG et al. Expression of X-linked inhibitor of apoptosis as a novel prognostic marker in radically resected non-small cell lung cancer patients. Clin Cancer Res.7, 2468-74 (2001)(PMID:11489828)
  3. Bilim V et al. Role of XIAP in the malignant phenotype of transitional cell cancer (TCC) and therapeutic activity of XIAP antisense oligonucleotides against multidrug-resistant TCC in vitro. Int J Cancer. 103, 29-37 (2003)(PMID:12455050)
  4. Muris JJ et al. Immunohistochemical profiling of caspase signaling pathways predicts clinical response to chemotherapy in primary nodal diffuse large B-cell lymphomas. Blood 105, 2916-23 (2005)(PMID:15576477)
  5. Cillessen SA et al. Inhibition of the intrinsic apoptosis pathway downstream of caspase-9 activation causes chemotherapy resistance in diffuse large B-cell lymphoma. Clin Cancer Res. 1, 7012-21 (2007)(PMID:18056177)
Product category
Research area
Apoptosis
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  • The availability is based on the information in Japan at 17:00, Aug 6, 2020 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.