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HOME > Product search results > Code No. M029-3 Anti-Caspase-4 (Human) mAb

Code No. M029-3

Anti-Caspase-4 (Human) mAb



Availability (in Japan)

10 or more

(In Japan at 17:00,
Aug 6, 2020 in JST)


100 µg/100 µL

  • Western Blotting

Clonality Monoclonal Clone 4B9
Isotype (Immunized Animal) Mouse IgG1 κ
1 μg/mL  
reported.  (PMID: 25943872
Immunogen (Antigen) Recombinant Human TX (N-terminal :1-270 a.a.)
Reactivity [Gene ID]


Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names CASP4, caspase 4, apoptosis-related cysteine peptidase, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II
Background The interleukin-1b converting enzyme (ICE)/CED-3 family proteases has been implicated in playing a fundamental role in programmed cell death. TX is a member of the ICE/CED-3 gene family encoding a cysteine protease that has a more than 50% sequence homology with ICE, especially in the region encoding the mature p20 and p10 ICE subunits and 30% sequence homology with Nedd-2/Ich-1L and CED-3. TX is able to cleave itself and the p30 ICE precursor and induces apoptosis in transfected cells1). TX is also a member of the caspase (CASP) family, CASP-4. An early biochemical event that occurs apoptosis in many cell types is the proteolytic cleavage of poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair. The several mammalian ICE homologues, ICE, TX, Nedd-2/Ich-1L and CPP32, are capable of cleaving PARP.
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Notes This antibody reacts with caspase-4 (43 kDa) on Western blotting using total cell lysate from U937, HL60 and HUC-Fm (Human primary cultured fibroblast), and also reacts with 44 kDa of myc-tagged-TX expressed in 293T cell. Occasionally, unidentified 68 kDa

Western Blotting

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  1. Flood B et al. Altered expression of caspases-4 and -5 during inflammatory bowel disease and colorectal cancer: Diagnostic and therapeutic potential. Clin Exp Immunol. 181, 39-50 (2015)(PMID:25943872)
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  • The availability is based on the information in Japan at 17:00, Aug 6, 2020 in JST.
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  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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