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HOME > Product search results > Code No. M029-3 Anti-Caspase-4 (Human) mAb

Code No. M029-3

Anti-Caspase-4 (Human) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 17:00,
Aug 6, 2020 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

Clonality Monoclonal Clone 4B9
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
WB
1 μg/mL  
IH*
reported.  (PMID: 25943872
Immunogen (Antigen) Recombinant Human TX (N-terminal :1-270 a.a.)
Reactivity [Gene ID]

Human[837]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names CASP4, caspase 4, apoptosis-related cysteine peptidase, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II
Background The interleukin-1b converting enzyme (ICE)/CED-3 family proteases has been implicated in playing a fundamental role in programmed cell death. TX is a member of the ICE/CED-3 gene family encoding a cysteine protease that has a more than 50% sequence homology with ICE, especially in the region encoding the mature p20 and p10 ICE subunits and 30% sequence homology with Nedd-2/Ich-1L and CED-3. TX is able to cleave itself and the p30 ICE precursor and induces apoptosis in transfected cells1). TX is also a member of the caspase (CASP) family, CASP-4. An early biochemical event that occurs apoptosis in many cell types is the proteolytic cleavage of poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair. The several mammalian ICE homologues, ICE, TX, Nedd-2/Ich-1L and CPP32, are capable of cleaving PARP.
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Notes This antibody reacts with caspase-4 (43 kDa) on Western blotting using total cell lysate from U937, HL60 and HUC-Fm (Human primary cultured fibroblast), and also reacts with 44 kDa of myc-tagged-TX expressed in 293T cell. Occasionally, unidentified 68 kDa
Citations

Western Blotting

  1. Lin XY et al. Expression analysis of the human caspase-1 subfamily reveals specific regulation of the CASP5 gene by lipopolysaccharide and interferon-gamma. J Biol Chem. 275, 39920-6 (2000)(PMID:10986288)
  2. Hitomi J et al. Involvement of caspase-4 in endoplasmic reticulum stress-induced apoptosis and Abeta-induced cell death. J Cell Biol. 165, 347-56 (2004)(PMID:15123740)
  3. Milleron RS, Bratton SB. Heat shock induces apoptosis independently of any known initiator caspase-activating complex. J Biol Chem. 281, 16991-17000 (2006)(PMID:16618700)
  4. Yukioka F et al. Presenilin-1 mutation activates the signaling pathway of caspase-4 in endoplasmic reticulum stress-induced apoptosis. Neurochem Int. 52, 683-7 (2008)(PMID:17942194)
  5. Oda T et al. Distinct mechanism of cell death is responsible for tunicamycin-induced ER stress in SK-N-SH and SH-SY5Y cells. Neurosci Res. 60, 29-39 (2008)(PMID:18029041)
  6. Koyama Y et al. Induction of amyloid beta accumulation by ER calcium disruption and resultant upregulation of angiogenic factors in ARPE19 cells. Invest Ophthalmol Vis Sci. 2008 49, 2376-83 (2008)(PMID:18515580)
  7. Pastorino JG, Shulga N. Tumor necrosis factor-alpha can provoke cleavage and activation of sterol regulatory element-binding protein in ethanol-exposed cells via a caspase-dependent pathway that is cholesterol insensitive. J Biol Chem. 283, 25638-49 (2008)(PMID:18635549)
  8. Nawrocki ST et al. Myc regulates aggresome formation, the induction of Noxa, and apoptosis in response to the combination of bortezomib and SAHA. Blood 112, 2917-26 (2008)(PMID:18641367)
  9. Nishitsuji K et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol. 174, 957-69 (2009)(PMID:19164507)
  10. Koyama Y et al. Familial amyotrophic lateral sclerosis (FALS)-linked SOD1 mutation accelerates neuronal cell death by activating cleavage of caspase-4 under ER stress in an in vitro model of FALS. Neurochem Int. 57, 838-43 (2010)(PMID:20816908)
  11. Hiratsuka T et al. Yokukansan inhibits neuronal death during ER stress by regulating the unfolded protein response. PLoS One 5, e13280 (2010)(PMID:20967273)
  12. Takemoto K et al. Mitochondrial TRAP1 regulates the unfolded protein response in the endoplasmic reticulum. Neurochem Int. 58, 880-7 (2011)(PMID:21338643)
  13. Huang TT et al. Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin. PLoS One 6, e18308 (2011)(PMID:21532751)
  14. Walsh JG et al. Caspase-1 promiscuity is counterbalanced by rapid inactivation of processed enzyme. J Biol Chem. 286, 32513-24 (2011)(PMID:2157759)
  15. Ito K et al. The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability. Protein Cell. 2, 755-63 (2011)(PMID:21976065)
  16. Yoshikawa T et al. Increased expression of tight junctions in ARPE-19 cells under endoplasmic reticulum stress. Curr Eye Res. 36, 1153-63 (2011)(PMID:21978097)
  17. Michallet AS et al. Compromising the unfolded protein response induces autophagy-mediated cell death in multiple myeloma cells. PLoS One 6, e25820 (2011)(PMID:22028791)
  18. Sollberger G et al. Caspase-4 is required for activation of inflammasomes. J Immunol. 188, 1992-2000 (2012)(PMID:22246630)
  19. Kajiwara Y et al. A critical role for human caspase-4 in endotoxin sensitivity. J Immunol. 193, 335-43 (2014)(PMID:24879791)
  20. Shi J et al. Inflammatory caspases are innate immune receptors for intracellular LPS. Nature 514, 187-92 (2014)(PMID:25119034)
  21. Flood B et al. Altered expression of caspases-4 and -5 during inflammatory bowel disease and colorectal cancer: Diagnostic and therapeutic potential. Clin Exp Immunol. 181, 39-50 (2015)(PMID:25943872)
  22. Gualdoni GA et al. Azithromycin inhibits IL-1 secretion and non-canonical inflammasome activation. Sci Rep. 5, 12016 (2015)(PMID:26152605)
  23. Aachoui Y et al. Canonical Inflammasomes Drive IFN-γγ to Prime Caspase-11 in Defense against a Cytosol-Invasive Bacterium. Cell Host Microbe. 18, 320-32 (2015)(PMID:26320999)
  24. Cheng X et al. Targeting autophagy enhances apatinib-induced apoptosis via endoplasmic reticulum stress for human colorectal cancer. Cancer Lett. 431, 105-114 (2018)(PMID:29859300)
  25. Platnich JM et al. Shiga Toxin/Lipopolysaccharide Activates Caspase-4 and Gasdermin D to Trigger Mitochondrial Reactive Oxygen Species Upstream of the NLRP3 Inflammasome. Cell Rep. 25 1525-1536 (2018)(PMID:30404007)
  26. Zasłona Z et al. Caspase-11 promotes allergic airway inflammation. Nat Commun. 11, 1055 (2020)(PMID:32103022)

Immunohistochemistry

  1. Flood B et al. Altered expression of caspases-4 and -5 during inflammatory bowel disease and colorectal cancer: Diagnostic and therapeutic potential. Clin Exp Immunol. 181, 39-50 (2015)(PMID:25943872)
Product category
Research area
Apoptosis
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  • The availability is based on the information in Japan at 17:00, Aug 6, 2020 in JST.
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  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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