HOME > Product search results > Code No. M025-3 Anti-Phospho-DNA Topoisomerase IIα (Thr1342) (Human) mAb

Code No.　M025-3

Anti-Phospho-DNA Topoisomerase IIα (Thr1342) (Human) mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 20, 2024 in JST）

Size

100 µL (1 mg/mL)

Data Sheet

SDS

Data	Immunocytochemistry
Clonality	Monoclonal	Clone	3D4
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB 1-10 µg/mL IC 10 µg/mL
Immunogen (Antigen)	Human Topo IIα (Thr1342) synthetic phosphopeptide corresponding to FSDFDEK(p)TDDEDFVPC (1335-1349 aa)
Reactivity [Gene ID]	Human, Mouse(-), Rat(-)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Background	Topoisomerase II (Topo II) is a nuclear enzyme that regulates the topological states of DNA by transient breakage and rejoining double-stranded DNA, catalyzing the decatenation and unknotting of topologically linked DNA circles and the relaxation of supercoiled DNA. In mammalian cells, Topo II consists of two isozymes, Topo IIα (170 kDa) and Topo IIβ (180 kDa). Expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo IIβ is expressed constantly throughout cell cycle, whereas the expression of Topo II&aplha; is cell cycle-regulated, peaking in G₂ to M phase and declining to a minimal level at the end of M phase. It is considered that Topo II&aplha; plays an essential role in cell proliferation, especially during late S to M phase. Threonine 1342 in human Topo II&aplha; is phosphorylated throughout the cell cycle. Phosphorylation level of threonine 1342 in G₂ to M phase is as twice as much in G₁ or S phase. Anti-phospho-DNA Topoisomerase IIα antibody recognize phosphorylated threonine residue, PT 1342 in human Topo IIα specifically and does not react with non-phosphorylated form.
Related products	M042-3 Anti-DNA Topoisomerase IIα (Human) mAb
Product category	Research area Cancer

The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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