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HOME > Product search results > Code No. D351-3 Anti-Reelin (Mouse) mAb

Code No. D351-3

Anti-Reelin (Mouse) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Dec 8, 2019 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

Clonality Monoclonal Clone 2F3
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
WB
1 µg/mL  
IH*
reported.  (PMID: 24644294
Immunogen (Antigen) KLH conjugated synthetic peptide, YEKPAFDYC (corresponding to amino acid residues 1241-1248 of mouse Reelin).
Reactivity [Gene ID]

Mouse[19699]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names Reln, reelin, rl, reeler
Background Reelinは、388 kDaからなる細胞外マトリックスの糖タンパク質であり、セリンプロテアーゼ活性を持つ。ReelinはApoER2/VLDLRの2つのReelin受容体と結合したのち,細胞内タンパク質であるDab1をリン酸化する。

Reelinの構造は、シグナル配列、SP配列に続いてReelin特異的なH配列が存在し、その後ろに300~350残基のアミノ酸配列が8回繰り返されるReelin repeatsで構成されている。リピート2と3の間のN-t cleavage、およびリピート6と7の間のC-t cleavageによって切断を受ける。本モノクローナル抗体は、R2切断部位をまたぐペプチドを免疫し、N-t cleavageを受けたReelinを認識しない抗体である。N-t cleavageによりReelinは不活性化されるため、本モノクローナル抗体は、活性型Reelinを特異的に認識するモノクローナル抗体である。

Reelinは、脳において神経細胞の移動や発達過程の脳の位置決定などに機能し、樹状突起、樹状突起棘の発達を促進する。Reelinの異常は脳構造の異常を導き、統合失調症や自閉症など神経変性疾患との関連も示唆されている。

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Citations

Western Blotting

  1. Koie M et al. Cleavage within Reelin repeat 3 regulates the duration and range of the signaling activity of Reelin protein. J Biol Chem. 289, 12922-30 (2015)(PMID:24644294)

Immunohistochemistry

  1. Koie M et al. Cleavage within Reelin repeat 3 regulates the duration and range of the signaling activity of Reelin protein. J Biol Chem. 289, 12922-30 (2015)(PMID:24644294)
Product category
Research area
Neuroscience
Brands
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Dec 8, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.