This site is for customers in Japan. Customers in other regions, please go to Global page.
HOME > Product search results > Code No. D147-3 Anti-Abi-1 (Abl interactor 1) mAb

Code No.　D147-3

Anti-Abi-1 (Abl interactor 1) mAb

Price

¥44,000

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 20, 2024 in JST）

Size

100 µL (1 mg/mL)

Data

  • Immunoprecipitation
Clonality Monoclonal Clone 1B9
Isotype (Immunized Animal) Mouse IgG1
Applications
WB
1 µg/mL  
IP
2 µg/200 µL of cell extract from 5x106 cells  
IC*
reported.  （PMID: 16280551 / 21464958 / 22808230）
IH
5 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
Immunogen (Antigen) Recombinant Human Abi-1 expressed in E.coli.
Reactivity [Gene ID]

Human[10006], Mouse[11308], Rat*[79249](PMID: 21464958/ 22102872), Hamster[100772463], Dog*[607247](PMID: 17164293)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names abl-interactor 1, E3B1, ABI-1, ABLBP4, NAP1BP, SSH3BP, SSH3BP1
Background Abl interactor (Abi) proteins were identified as a c-Abl-binding protein that bind to both the Src homology 3 (SH3) domain and the C-terminal proline-rich regions of Abl kinase through dual SH3-PXXP interactions. Two highly related genes, abi-1 and abi-2, were cloned. In addition to the interaction with Abl kinase, Abi proteins also interact with other signaling molecules. Recently, it has been reported that Abi-1 dramatically promoted c-Abl-mediated tyrosine (Tyr296) phosphorylation of Mena [mammalian homologue of Drosophila Enabled (Ena)] by interacting with both proteins.
Citations

Western Blotting

  1. OdaA et al. WAVE/Scars in platelets. Blood 105, 3141-8 (2005)(PMID:15280206)
  2. Kheir WA et al. A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages. J Cell Sci. 118, 5369-79 (2005)(PMID:16280551)
  3. Yamazaki D et al. Rac-WAVE-mediated actin reorganization is required for organization and maintenance of cell-cell adhesion. J Cell Sci. 120, 86-100 (2007)(PMID:17164293)
  4. Eto K et al. The WAVE2/Abi1 complex differentially regulates megakaryocyte development and spreading: implications for platelet biogenesis and spreading machinery. Blood 110, 3637-47 (2007)(PMID:17664349)
  5. Liebau S et al. An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis. PLoS One. 6, e18148 (2011)(PMID:21464958)
  6. Proepper C et al. Heterogeneous nuclear ribonucleoprotein k interacts with Abi-1 at postsynaptic sites and modulates dendritic spine morphology. PLoS One. 6, e27045 (2011)(PMID:22102872)
  7. Steinestel K et al. Expression of Abelson interactor 1 (Abi1) correlates with inflammation, KRAS mutation and adenomatous change during colonic carcinogenesis. PLoS One. 7, e40671 (2012)(PMID:22808230)
  8. Xiong X et al. Disruption of Abi1/Hssh3bp1 expression induces prostatic intraepithelial neoplasia in the conditional Abi1/Hssh3bp1 KO mice. Oncogenesis 1, e26 (2012)(PMID:23552839)
  9. Steinestel K et al. Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells. Mol Cancer. 13, 145 (2014)(PMID:24913355)
  10. Yu X et al. Inhibitory short peptides targeting EPS8/ABI1/SOS1 tri-complex suppress invasion and metastasis of ovarian cancer cells. BMC Cancer. 19, 878 (2019)(PMID:31488087)
  11. Qi Y et al. PTEN suppresses epithelial-mesenchymal transition and cancer stem cell activity by downregulating Abi1. Sci Rep. 10, 12685 (2020) (PMID:32728066)

Immunoprecipitation

  1. OdaA et al. WAVE/Scars in platelets. Blood 105, 3141-8 (2005)(PMID:15280206)
  2. Kheir WA et al. A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages. J Cell Sci. 118, 5369-79 (2005)(PMID:16280551)
  3. Sekino S et al. The NESH/Abi-3-based WAVE2 complex is functionally distinct from the Abi-1-based WAVE2 complex. Cell Commun Signal. 13, 41 (2015)(PMID:26428302)

Immunocytochemistry

  1. Kheir WA et al. A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages. J Cell Sci. 118, 5369-79 (2005)(PMID:16280551)
  2. Liebau S et al. An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis. PLoS One. 6, e18148 (2011)(PMID:21464958)
  3. Steinestel K et al. Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells. Mol Cancer. 13, 145 (2014)(PMID:24913355)

Immunohistochemistry

  1. Steinestel K et al. Expression of Abelson interactor 1 (Abi1) correlates with inflammation, KRAS mutation and adenomatous change during colonic carcinogenesis. PLoS One. 7, e40671 (2012)(PMID:22808230)
  2. Steinestel K et al. Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells. Mol Cancer. 13, 145 (2014)(PMID:24913355)
Product category
Research area
Cancer
Signal transduction
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 17:00, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.