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HOME > Product search results > Code No. D048-3 Anti-IL-18 (Mouse) mAb

Code No. D048-3

Anti-IL-18 (Mouse) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 17:00,
Aug 14, 2020 in JST)

Size

100 µg/100 µL

Data
  • Immunoprecipitation

Clonality Monoclonal Clone 93-10C
Isotype (Immunized Animal) Rat IgG1
Applications
IP
5 µg/0.5 µg of recombinant mouse IL-18  
NT
0.5-5 µg/mL  
Immunogen (Antigen) Recombinant Mouse IL-18
Reactivity [Gene ID]

Mouse[16173]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names interleukin 18 (interferon-gamma-inducing factor), IGIF, IL1F4, IL-1g, IL-18
Background Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-g (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-g. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
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Citations

Neutralization

  1. Fukao T et al. Synergistic effects of IL-4 and IL-18 on IL-12-dependent IFN-gamma production by dendritic cells. 164, 64-71 (2000)(PMID:10604994)
  2. Malmgaard L, Paludan SR. Interferon (IFN)-alpha/beta, interleukin (IL)-12 and IL-18 coordinately induce production of IFN-gamma during infection with herpes simplex virus type 2. J Gen Virol. 84, 2497-500 (2003)(PMID:12917471)
  3. Wang SZ et al. IL-12p40 and IL-18 modulate inflammatory and immune responses to respiratory syncytial virus infection. J Immunol. 173, 4040-9 (2004)(PMID:15356153)
  4. Beadling C, Slifka MK. Differential regulation of virus-specific T-cell effector functions following activation by peptide or innate cytokines. Blood 105, 1179-86 (2005)(PMID:15471952)
  5. Chaix J et al. Cutting edge: Priming of NK cells by IL-18. J Immunol. 181, 1627-31 (2008)(PMID:18641298)
  6. Humann J, Lenz LL. Activation of naive NK cells in response to Listeria monocytogenes requires IL-18 and contact with infected dendritic cells. J Immunol. 184, 5172-8 (2010)(PMID:20351186)
  7. Matsunaga H et al. Physiological stress exacerbates murine colitis by enhancing proinflammatory cytokine expression that is dependent on IL-18. Am J Physiol Gastrointest Liver Physiol. 301, G555-64 (2011)(PMID:21719737)
  8. Kahlenberg JM et al. Neutrophil extracellular trap-associated protein activation of the NLRP3 inflammasome is enhanced in lupus macrophages. J Immunol. 190, 1217-26 (2013)(PMID:23267025)
  9. Chen JM et al. IL-18 induced IL-23/IL-17 expression impairs Aβ clearance in cultured THP-1 and BV2 cells. Cytokine. 119, 113-118 (2019)(PMID:30903865)

Dot Blotting

  1. Matsunaga H et al. Physiological stress exacerbates murine colitis by enhancing proinflammatory cytokine expression that is dependent on IL-18. Am J Physiol Gastrointest Liver Physiol. 301, G555-64 (2011)(PMID:21719737)
Product category
Research area
Immunology
Cytokine & Growth Factors
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 17:00, Aug 14, 2020 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.