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HOME > Product search results > Code No. D047-3 Anti-IL-18 (Mouse) mAb

Code No. D047-3

Anti-IL-18 (Mouse) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Nov 16, 2019 in JST)

Size

100 µg/100 µL

Data
  • Immunoprecipitation

Clonality Monoclonal Clone 74
Isotype (Immunized Animal) Rat IgG2a
Applications
IP
5 μg/0.5 μg Mouse IL-18  
Immunogen (Antigen) Recombinant Mouse IL-18
Reactivity [Gene ID]

Mouse[16173]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names interleukin 18 (interferon-gamma-inducing factor), IGIF, IL1F4, IL-1g, IL-18
Background Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
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Citations

Western Blotting

  1. Luo Y et al. Recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG) expressing mouse IL-18 augments Th1 immunity and macrophage cytotoxicity. Clin Exp Immunol. 137, 24-34 (2004)(PMID:15196240)
  2. Murphy AJ et al. IL-18 Production from the NLRP1 Inflammasome Prevents Obesity and Metabolic Syndrome. Cell Metab. 23, 155-64 (2016)(PMID:26603191)

ELISA

  1. Abu Elhija M et al. Over-expression of IL-18, ICE and IL-18 R in testicular tissue from sexually immature as compared to mature mice. Eur Cytokine Netw. 19, 15-24 (2008)(PMID:18299270)
  2. Abu Elhija M et al. LPS increases the expression levels of IL-18, ICE and IL-18 R in mouse testes. Am J Reprod Immunol. 2008 60, 361-71 (2008)(PMID:19046143)
  3. Wang X et al. The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation. Infect Immun. 84, 1796-1805 (2016)(PMID: 27045038)
  4. Wang X et al. The Prostaglandin E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4 Inflammasome Activation. PLoS Pathog. 12, e1005803 (2016)(PMID:27482714)
  5. Weindel CG et al. Autophagy in Dendritic Cells and B Cells Is Critical for the Inflammatory State of TLR7-Mediated Autoimmunity. J Immunol. 198, 1081-1092 (2017) (PMID:28031336)
  6. Lasseaux C et al. Type I interferons drive inflammasome-independent emergency monocytopoiesis during endotoxemia. Sci Rep. 7, 16935 (2017)(PMID:29209091)
  7. Nichols RD et al. NAIP/NLRC4 inflammasome activation in MRP8+ cells is sufficient to cause systemic inflammatory disease. Nat Commun. 8, 2209 (2017)(PMID:29263322)
  8. Flood B et al. Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis. Oncogene. 38, 2658-2674 (2019)(PMID:30538296)
Product category
Research area
Immunology
Cytokine & Growth Factors
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  • The availability is based on the information in Japan at 00:05, Nov 16, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.