Anti-HP1α pAb

Price

¥49,500

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 25, 2024 in JST）

Size

100 µL (500 µg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit IgG
Applications	WB 0.25-1 µg/mL IP 1 µg/200 µL of cell extract from 5x10⁶ cells IC 10 µg/mL IH 10 µg/mL (Heat treatment is necessary for paraffin embedded sections.) ChIP* reported. （PMID: 24561205）
Immunogen (Antigen)	synthetic peptide (CHAYPEDAENKEKETAKS) (Human HP1alpha, 175-191 a.a.)
Reactivity [Gene ID]	Human[23468], Mouse[12419], Rat(-)
Storage buffer	500 µg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	chromobox 5, CBX5, HP1A, HEL25
Related products	BMP002 Anti-HP1β pAb BMP003 Anti-HP1γ pAb
Citations	Western Blotting Kashiwagi K et al. DNA methyltransferase 3b preferentially associates with condensed chromatin. Nucleic Acids Res. 39, 874-88 (2011)(PMID:20923784) Okumura F et al. TRIM8 regulates Nanog via Hsp90β-mediated nuclear translocation of STAT3 in embryonic stem cells. Biochim Biophs Acta. 1813, 1784-92 (2011)(PMID:21689689) Shiomi Y, Nishitani H. Alternative replication factor C protein, Elg1, maintains chromosome stability by regulating PCNA levels on chromatin. Genes Cells. 18, 946-59 (2013)(PMID:23937667) Nishibuchi G et al. N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity. Nucleic Acids Res. 42, 12498-511 (2014)(PMID:25332400) ChIP Shen WY et al. CTCF-mediated reduction of vigilin binding affects the binding of HP1α to the satellite 2 locus. FEBS Lett. 588, 1549-55 (2014)(PMID:24561205)
Product category	Research area Epigenetics

The availability is based on the information in Japan at 17:00, Apr 25, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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