Western blot analysis of exosomes isolated from various samples
Detection of miR-21 by using qRT-PCR protocol for ExoCap™ Streptavidin Kit
||MEX-SA: Streptavidin Magnetic Beads (2 mL), Treatment Buffer (30 mL), Washing/Dilution Buffer (60 mL) MEX123-60:Anti-CD9 mAb-Biotin (10 µg/50 µL), Anti-CD63 (LAMP-3) mAb-Biotin (10 µg/50 µL), Anti-CD81 (TAPA1) mAb-Biotin (10 µg/50 µL)
||ExoCap™ Streptavidin CD9/CD63/CD81 Set allows for exosome isolation from serum, plasma and cell culture supernatant. Isolated exosomes can be used for western blotting, beads CLEIA, flow cytometry and qRT-PCR.
||Serum, plasma and cell-cultured supernatant
||ExoCap™ Streptavidin CD9/CD63/CD81 Set contains ExoCap™ Streptavidin Kit and Exosome CD9/CD63/CD81 mAb Set-Biotin. This set is designed for isolation and analysis of exosomes or microvesicles, called “Extracellular Vesicles (EVs)”, using biotinylated antibodies against exosome surface marker CD9, CD63 and CD81. Exosomes are extracellular vesicles secreted by most cell types and contain various marker proteins and RNAs, such as microRNA and fragmented mRNA. ExoCap™ Streptavidin Kit uses functionalized Magnosphere™ magnetic microparticles for exosome isolation. These beads are coated with a JSR Life Sciences proprietary hydrophilic polymer to decrease non-specific binding.
||MEX-SA ExoCap™ Streptavidin Kit
MEX123-60 Exosome CD9/CD63/CD81 mAb Set-Biotin
MEX001-6 Anti-CD9 mAb-Biotin
MEX002-6 Anti-CD63 (LAMP-3) mAb-Biotin
MEX003-6 Anti-CD81 (TAPA1) mAb-Biotin
MEX004-6 Anti-CD326 (EpCAM) mAb-Biotin
M075-6 Mouse IgG1 (isotype control)-Biotin
M076-6 Mouse IgG2a (isotype control)-Biotin
M077-6 Mouse IgG2b (isotype control)-Biotin
M078-6 Mouse IgG3 (isotype control)-Biotin
MEX-E ExoCap™ Nucleic Acid Elution Buffer
MEX1001 ExoDiluent for Immunoassay
||ExoCap™ Streptavidin CD9/CD63/CD81 Set contains ExoCap™ Streptavidin Kit and Exosome CD9/CD63/CD81 mAb Set-Biotin.
- Research area