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HOME > Product search results > Code No. K0215-3 Anti-HLA-E (Human) mAb

Code No. K0215-3

Anti-HLA-E (Human) mAb

Price

¥30,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Aug 22, 2019 in JST)

Size

100 µg/100 µL

Data
  • Flow Cytometry

Clonality Monoclonal Clone 4D12
Isotype (Immunized Animal) Mouse IgG1
Applications
FCM
10 µg/mL (final concentration)  
IH*
reported.  (PMID: 20947660
Immunogen (Antigen) This antibody was established by immunization of HLA-B27 transgenic mice with HLA-E protein.
Reactivity [Gene ID]

Human

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background HLA-E (human leucocyte antigen-E) is a conserved class I major histocompatibility molecule. It binds to the leader peptide derived from the polymorphic classical MHC molecules HLA-A, HLA-B and HLA-C. This peptide binding stabilizes the HLA-E protein and allows it to migrate to the cell surface. HLA-E then interacts with CD94/NKG2A receptors on natural killer cells. This interaction inhibits natural killer cell-mediated lysis of cells displaying HLA-E. In virally infected or tumor cells, down-regulation of HLA-A, HLA-B and HLA-C production prevents stabilization of HLA-E by the leader peptide. Under these circumstances, HLA-E is degraded before it reaches the cell surface and the cell is then vulnerable to lysis by natural killer cells.
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Citations

Flow Cytometry

  1. Nguyen S et al. HLA-E upregulation on IFN-gamma-activated AML blasts impairs CD94/NKG2A-dependent NK cytolysis after haplo-mismatched hematopoietic SCT. Bone Marrow Transplant. 43, 693-9 (2009)(PMID:19011664)
  2. Tsukamoto N et al. Impairment of plasmacytoid dendritic cells for IFN production by the ligand for immunoglobulin-like transcript 7 expressed on human cancer cells. Clin Cancer Res. 15, 5733-43 (2009)(PMID:19723650)

Immunohistochemistry

  1. Cui CH et al. Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy. Hum Mol Genet. 20, 235-44 (2011)(PMID:20947660)
Product category
Research area
Immunology
Cell surface antigens
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Aug 22, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.