CycLex Cellular BrdU ELISA Kit is a non-isotopic immunoassay
used for the semi-quantitative measurement of BrdU (bromodeoxyuridine) incorporation in newly
synthesized DNA during DNA synthesis.
A rapid and convenient method for estimating S-phase cells in a population was developed which detects bromodeoxyuridine (BrdU) incorporation into DNA by means of monoclonal anti-BrdU antibodies in a cellular ELISA format. It has been shown that a precise evaluation of cell proliferation could be performed by the measurement of BrdU incorporation in newly synthesized cellular DNA. In addition, there is a good correlation between the cellular BrdU ELISA and the [3H]-thymidine incorporation assay as shown for a variety of murine and human cell systems, including mitogen- and antigen-stimulated lymphocytes and cytokine-induced proliferation of different cell lines.
CycLex Histone H2A.X Phosphorylation Cellular ELISA Kit is a
non-isotopic immunoassay used for the semi-quantitative measurement of histone H2A.X
phosphorylation level in response to DNA double strand break in situ by means of cell-based ELISA.
Histone H2A.X has been implicated in the maintenance of genomic stability in response to DNA double strand breaks (DSBs). It is phosphorylated at an evolutionary conserved PI3 kinase related kinase motif in the carboxyl terminus within seconds after exposure to ionizing radiation (IR). Immunofluorescence studies have revealed that phosphorylated H2AX (γ-H2AX) forms nuclear foci at the sites of DSBs. These foci appear within 1 min after exposure of cells to IR. Their numbers increase in the first 10-30 min after irradiation before they gradually decline correlating with the predicted value of slowly re-joining DSBs. γ-H2AX foci are also found at sites of V(D)J recombination-induced DSBs in developing thymocytes and at sites of recombinational DSBs during meiosis. In addition, phosphorylation of H2AX is also induced by initiation of DNA fragmentation during apoptosis. Thus, H2AX is phosphorylated in response to DSBs.