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HOME > Product search results > Code No. D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb

Code No. D289-3

Anti-Np95 (Uhrf1) (Mouse) mAb

Availability (in Japan)

1-9

(In Japan at 01:30,
Nov 24, 2017 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

  • Immunocytochemistry

Clonality Monoclonal Clone Th-10a
Isotype (Immunized Animal) Rat IgG2aκ
Applications
WB
1 µg/mL  
IP*
reported.  (PMID: 8634372
FCM*
reported.  (PMID: 8634372
IC
2 µg/mL  
IH*
reported.  (PMID: 8634372
Immunogen (Antigen) Mouse thymic lymphoma
Reactivity [Gene ID]

Human(-), Mouse[18140], Rat(-), Hamster(-)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names ICBP90, RNF106, AL022808
Background DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs that appear after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, a tandem Tudor domain, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
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Citations

Western Blotting

  1. Bonapace IM et al. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. J Cell Biol. 157, 909-14 (2002)(PMID:12058012)
  2. Muto M et al. Targeted disruption of Np95 gene renders murine embryonic stem cells hypersensitive to DNA damaging agents and DNA replication blocks. J Biol Chem. 277, 34549-55 (2002)(PMID:12084726)
  3. Fujimori A et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mamm Genome. 9, 1032-5 (1998)(PMID:9880673)

Immunoprecipitation

  1. Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995)(PMID:8634372)

Flow Cytometry

  1. Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995)(PMID:8634372)

Immunocytochemistry

  1. Bonapace IM et al. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. J Cell Biol. 157, 909-14 (2002)(PMID:12058012)
  2. Sharif J et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature. 450, 908-12 (2007)(PMID:17994007)
  3. Uemura T et al. Temporal and spatial localization of novel nuclear protein NP95 in mitotic and meiotic cells. Cell Struct Funct. 25, 149-59 (2000)(PMID:10984098)
  4. Ohno R et al. A replication-dependent passive mechanism modulates DNA demethylation in mouse primordial germ cells. Development 140, 2892-903 (2013)
     (PMID:23760957)

Immunohistochemistry

  1. Fujimori A et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mamm Genome. 9, 1032-5 (1998)(PMID:9880673)
  2. Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995)(PMID:8634372)
Product category
Research area
Epigenetics
Cancer
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  • The availability is based on the information in Japan at 01:30, Nov 24, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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