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HOME > Product search results > Code No. D162-3 Anti-Flavocytochrome b558 (Human) mAb

Code No. D162-3

Anti-Flavocytochrome b558 (Human) mAb

Availability (in Japan)

10 or more

(In Japan at 01:30,
Dec 13, 2017 in JST)

Size

100 µg/100 µL

Data
  • Flow Cytometry

Clonality Monoclonal Clone 7D5
Isotype (Immunized Animal) Mouse IgG1
Applications
FCM
5 µg/mL (final concentration)  
Immunogen (Antigen) Human cytochrome b rich fraction
Reactivity [Gene ID]

Human[1535/1536]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names CYBA, p22-PHOX, CYBB, CGD, NOX2, IMD34, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX
Background The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2-), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase.  It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2-.  Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.
Related products D162-4 Anti-Flavocytochrome b558 (Human) mAb-FITC
D162-5 Anti-Flavocytochrome b558 (Human) mAb-PE
Citations

Flow Cytometry

  1. Li XJ et al. Leu505 of Nox2 is crucial for optimal p67phox-dependent activation of the flavocytochrome b558 during phagocytic NADPH oxidase assembly. J Leukoc Biol. 81, 238-49 (2007)(PMID:17060362)
  2.  Debeurme F et al. Regulation of NADPH oxidase activity in phagocytes: relationship between FAD/NADPH binding and oxidase complex assembly. 285, 33197-208 (2010)
     
    (PMID:25374740)
  3. Lugo Reyes SO et al. Variant of X-Linked Chronic Granulomatous Disease Revealed by a Severe Burkholderia cepacia Invasive Infection in an Infant. Case Reports Immunol. 2013, 323614 (2013)(PMID:25374740)
  4. Baba LA et al. Chronic granulomatous disease in Morocco: genetic, immunological, and clinical features of 12 patients from 10 kindreds. J Clin Immunol. 34, 452-8 (2014)(PMID:24596025)
Product category
Research area
Signal transduction
Cellular stress
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Dec 13, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.