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HOME > Product search results > Code No. M114-3 Anti-SUMO-2/3 mAb

Code No. M114-3

Anti-SUMO-2/3 mAb

Availability (in Japan)

10 or more

(In Japan at 00:05,
Jul 19, 2018 in JST)

Size

100 µg/100 µL

This antibody reacts with SUMO-2/3 (15 kDa) but not react with SUMO-1 (15~17 kDa) on Western blotting.
Data
  • Western Blotting

  • Immunocytochemistry

Clonality Monoclonal Clone 1E7
Isotype (Immunized Animal) Mouse IgG2b
Applications
WB
2 μg/mL  
IC
5 μg/mL  
Immunogen (Antigen) Recombinant Human full length SUMO-3
Reactivity [Gene ID]

Human[6613/6612], Mouse[170930/20610], Rat[690244/499417]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names SUMO2, SUMO3, HSMT3, SMT3A, SMT3B, Smt3A, Smt3B, SMT3H1, SMT3H2
Background Sumoylation, the covalent attachment of a small ubiquitin-like modifier (SUMO) peptide to lysine residues of targeted substrate, has recently emerged as an important mechanism in transcriptional control. In humans and mice, the SUMO family consists of three members, SUMO-1, -2, and -3. SUMO-2 (SMT3A, Sentrin-3) and SUMO-3 (SMT3B, Sentrin-2) are similar (~95% identical), but less closely related to SUMO-1 (~50% identical). Whereas many proteins modified by SUMO-1 were identified, many as yet unidentified proteins are modified by SUMO-2/3 after exposure of cells to various stress stimuli.
Related products M113-3 Anti-SUMO-1 mAb
M114-A48 Anti-SUMO-2/3 (Human) mAb-Alexa Fluor® 488
CM003-3 Anti-CHIP mAb
D058-3 Anti-Multi Ubiquitin mAb
MK-12-3 Anti-Ubiquitin mAb
Citations

Western Blotting

  1. Klein UR et al. RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin. Mol. Biol. Cell 20, 410-8 (2009)(PMID:18946085)
  2. Du JX et al. A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4. J biol. Chem. 285, 28298-308 (2010)(PMID:20584900)
  3. Fernández-Miranda G et al. SUMOylation modulates the function of Aurora-B kinase. J Cell Sci. 123, 2823-33 (2010)(PMID:20663916)
  4. Finkbeiner E et al. The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex. EMBO J. 30, 1067-78 (2011)(PMID:21326211)
  5. Fanis P et al. Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. Mol Cell Proteomics 11, 1263-73 (2012)(PMID:23122649)
  6. Psakhye I, Jentsch S. Protein group modification and synergy in the SUMO pathway as exemplified in DNA repair. Cell 151, 807-20 (2012)(PMID:23122649)
  7. Guo C et al. SENP3-mediated deSUMOylation of dynamin-related protein 1 promotes cell death following ischaemia. EMBO J. 32, 1514-28 (2013)(PMID:23524851)
  8. Berndt A et al. In vivo characterization of the properties of SUMO1-specific monobodies. Biochem J. 456, 385-95 (2013)(PMID:24040933)
Product category
Research area
Ubiquitin proteasome
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  • The availability is based on the information in Japan at 00:05, Jul 19, 2018 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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