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HOME > Product search results > Code No. D071-3 Anti-Multi Ubiquitin mAb

Code No. D071-3

Anti-Multi Ubiquitin mAb

Availability (in Japan)

10 or more

(In Japan at 00:05,
Nov 22, 2018 in JST)

Size

100 µg/100 µL

Clone FK1 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono ubiquitinylated proteins but not free ubiquitin (PMID:14976165, 24805825) .
Data
  • Western Blotting

Clonality Monoclonal Clone FK1
Isotype (Immunized Animal) Mouse IgM
Applications
WB
1-5 µg/mL  
Immunogen (Antigen) a crude poly-ubiquitin-lysozyme
Reactivity [Gene ID]

Human, Mouse*(24124537)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
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Citations

Western Blotting

  1. Seino H et al. Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin. Mol Cell Biol. 23, 3497-3505 (2003)(PMID:12724408)
  2. Ledda F et al. Lrig1 is an endogenous inhibitor of Ret receptor tyrosine kinase activation, downstream signaling, and biological responses to GDNF. J Neurosci. 28, 39-49 (2008)(PMID:18171921)
  3. Umebayashi K et al. Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation. Mol Biol Cell. 19, 3454-3462 (2008)(PMID:18508924)
  4. Waguri S, Komatsu M. Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice. Methods Enzymol. 453, 181-196 (2009)(PMID:19216907)
  5. Zhou L, Yang H. The von Hippel-Lindau tumor suppressor protein promotes c-Cbl-independent poly-ubiquitylation and degradation of the activated EGFR. PLoS One. 6, e23936 (2011)(PMID:21949687)
  6. Nivon M et al. NF-κB regulates protein quality control after heat stress through modulation of the BAG3-HspB8 complex. J Cell Sci. 125, 1141-51 (2012)(PMID:22302993)
  7. Pizon V et al. A MURF2B, a novel LC3-binding protein, participates with MURF2A in the switch between autophagy and ubiquitin proteasome system during differentiation of C2C12 muscle cells. PLoS One. 8, e76140 (2013)(PMID:24124537)
  8. Carrano AC et al. A Krüppel-like factor downstream of the E3 ligase WWP-1 mediates dietary-restriction-induced longevity in Caenorhabditis elegans. Nat Commun. 5, 3772 (2014)(PMID:24805825)
  9. Yahiro K et al. Helicobacter pylori VacA induces apoptosis by accumulation of connexin 43 in autophagic vesicles via a Rac1/ERK-dependent pathway. Cell Death Dis. 1, 15035 (2015)(PMID:27551466)
References
  1. Morris JR, Solomon E. BRCA1 : BARD1 induces the formation of conjugated ubiquitin structures, dependent on K6 of ubiquitin, in cells during DNA replication and repair. Hum Mol Genet. 13, 807-17 (2004)(PMID: 14976165
Product category
Research area
Ubiquitin proteasome
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Nov 22, 2018 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.