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HOME > Product search results > Code No. D058-8 Anti-Multi Ubiquitin mAb-Agarose

Code No. D058-8

Anti-Multi Ubiquitin mAb-Agarose

Availability (in Japan)

10 or more

(In Japan at 00:05,
Sep 20, 2018 in JST)

Size

Gel: 200 µL

Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono ubiquitinylated proteins but not free ubiquitin (PMID:18757370, 19237541) .
Data
  • Immunoprecipitation

Clonality Monoclonal Clone FK2
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
IP
20 µL of gel slurry/300 µL of cell extract from 3x106 cells  
Immunogen (Antigen) a crude poly-ubiquitin-lysozyme
Reactivity [Gene ID]

Human

Storage buffer 500-600 µg of anti-Multi Ubiquitin monoclonal antibody covalently coupled to 200 µL of agarose gel and provided as a 50% gel slurry (in PBS/0.1% ProClin 150)
Storage temp. 4°C Conjugate Agarose Manufacturer MBL
Background Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
Related products MK-11-3 Anti-Ubiquitin mAb
D058-3 Anti-Multi Ubiquitin mAb
M075-8 Mouse IgG1 (isotype control)-Agarose
MK-12-3 Anti-Ubiquitin mAb
D058-11 Anti-Multi Ubiquitin mAb-Magnetic Beads
Citations

Immunoprecipitation

  1. Karunadharma PP et al. Respiratory chain protein turnover rates in mice are highly heterogeneous but strikingly conserved across tissues, ages, and treatments. FASEB J. 29, 3582-92 (2015)(PMID:25977255)
  2. Gainullin MR et al. Degradation of cofilin is regulated by Cbl, AIP4 and Syk resulting in increased migration of LMP2A positive nasopharyngeal carcinoma cells. Sci Rep. 7, 9012 (2017)(PMID:28827787)
  3. Gastou M et al. The severe phenotype of Diamond-Blackfan anemia is modulated by heat shock protein 70. Blood Adv. 1, 1959-1976 (2017)(PMID:29296843)
Product category
Research area
Ubiquitin proteasome
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  • The availability is based on the information in Japan at 00:05, Sep 20, 2018 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.