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HOME > Product search results > Code No. D058-3 Anti-Multi Ubiquitin mAb

Code No. D058-3

Anti-Multi Ubiquitin mAb

Availability (in Japan)

10 or more

(In Japan at 00:05,
Sep 20, 2018 in JST)

Size

100 µg/100 µL

Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono ubiquitinylated proteins but not free ubiquitin (PMID:18757370, 19237541) .
Data
  • Western Blotting

Clonality Monoclonal Clone FK2
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
WB
1-5 µg/mL  
IC*
reported.  (PMID: 12860994 / 15858004 / 16716190
IH*
reported.  (PMID: 29097807 / 29282717
ELISA*
reported.  (PMID: 7588772
Immunogen (Antigen) a crude poly-ubiquitin-lysozyme
Reactivity [Gene ID]

Human, Mouse*(15975924/17114435/18083104), Monkey*(15858004), Yeast*(12724408/15998872)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
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Citations

Western Blotting

  1. Yokosawa N et al. C-terminal region of STAT-1alpha is not necessary for its ubiquitination and degradation caused by mumps virus V protein. J Virol. 76, 12683-90 (2002)(PMID:12438594)
  2. Seino H et al. Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin. Mol Cell Biol. 23, 3497-3505 (2003)(PMID:12724408)
  3. Inukai N et al. A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. J Biol Chem. 279, 8190-5 (2004)(PMID:14662762)
  4. Hwang GW et al. Overexpression of Rad23 confers resistance to methylmercury in saccharomyces cerevisiae via inhibition of the degradation of ubiquitinated proteins. Mol Pharmacol. 68, 1074-8 (2005)(PMID:15998872)
  5. Yamashita M et al. Ras-ERK MAPK cascade regulates GATA3 stability and Th2 differentiation through ubiquitin-proteasome pathway. J Biol Chem. 280, 29409-19 (2005)(PMID:15975924)
  6. Masuda Y et al. ADRP/adipophilin is degraded through the proteasome-dependent pathway during regression of lipid-storing cells. J Lipid Res. 47, 87-98 (2006)(PMID:16230742)
  7. Hosokawa H et al. Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3. J Immunol. 177, 7656-64 (2006)(PMID:17114435)
  8. Komatsu M et al. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131, 1149-63 (2007)(PMID:18083104)
  9. Shi W et al. Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation. J Biol Chem. 283, 31608-16 (2008)(PMID:18757370)
  10. Gitcho MA et al. VCP mutations causing frontotemporal lobar degeneration disrupt localization of TDP-43 and induce cell death. J Biol Chem. 284, 12384-98 (2009)(PMID:19237541)
  11. Ebina M et al. Myeloma overexpressed 2 (Myeov2) regulates L11 subnuclear localization through Nedd8 modification. PLoS One. 8, e65285 (2013)(PMID:23776465)
  12. Furuya N et al. PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation. Autophagy 10, 631-41 (2014)(PMID:24451648)
  13. Ogawa M et al. Impaired O-linked N-acetylglucosaminylation in the endoplasmic reticulum by mutated epidermal growth factor (EGF) domain-specific O-linked N-acetylglucosamine transferase found in Adams-Oliver syndrome. J Biol Chem. 290, 2137-49 (2015)(PMID:25488668)
  14. Sin Y et al. The C-terminal region and SUMOylation of cockayne syndrome group B protein play critical roles in transcription-coupled nucleotide excision repair. J Biol Chem. 291, 1387-97 (2016)(PMID:26620705)
  15. Takayama Y et al. Dissection of ubiquitinated protein degradation by basal autophagy. FEBS Lett. 591, 1199-1211 (2017)(PMID:28369861)
  16. Okuda K et al. CDKL5 controls postsynaptic localization of GluN2B-containing NMDA receptors in the hippocampus and regulates seizure susceptibility. Neurobiol Dis. 106, 158-170 (2017)(PMID:28688852)
  17. Kim J et al. KLHL7 promotes TUT1 ubiquitination associated with nucleolar integrity: Implications for retinitis pigmentosa. Biochem Biophys Res Commun. 494, 220-226 (2017)(PMID:29032201)
  18. Mitsuhashi H et al. Functional domains of the FSHD-associated DUX4 protein. Biol Open 7, bio033977 (2018)(PMID:29618456)

Immunocytochemistry

  1. Yokosawa N et al. C-terminal region of STAT-1alpha is not necessary for its ubiquitination and degradation caused by mumps virus V protein. J Virol. 76, 12683-90 (2002)(PMID:12438594)
  2. Katoh K et al. The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting. J Biol Chem. 278, 39104-13 (2003)(PMID:12860994)
  3. Broering TJ et al. Carboxyl-proximal regions of reovirus nonstructural protein muNS necessary and sufficient for forming factory-like inclusions. J Virol. 79, 6194-6206 (2005)(PMID:15858004)
  4. Nakamura M et al. Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes. Genes Cells 11, 593-606 (2006)(PMID:16716190)
  5. Ishioka T et al. Impairment of the ubiquitin-proteasome system by cellular FLIP. Genes Cells 12, 735-44 (2007)(PMID:17573774)
  6. Komatsu M et al. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131, 1149-63 (2007)(PMID:18083104)
  7. Shi W et al. Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation. J Biol Chem. 283, 31608-16 (2008)(PMID:18757370)
  8. Gitcho MA et al. VCP mutations causing frontotemporal lobar degeneration disrupt localization of TDP-43 and induce cell death. J Biol Chem. 284, 12384-98 (2009)(PMID:19237541)
  9. Choi UY et al. Polyubiquitin chain-dependent protein degradation in TRIM30 cytoplasmic bodies. Exp Mol Med. 47, e159 (2015)(PMID:25882191)
  10. Ohka N et al. SNIPER(TACC3) induces cytoplasmic vacuolization and sensitizes cancer cells to Bortezomib. Cancer Sci. 108, 1032-1041 (2017)(PMID:28192613)
  11. Takayama Y et al. Dissection of ubiquitinated protein degradation by basal autophagy. FEBS Lett. 591, 1199-1211 (2017)(PMID:28369861)

Immunohistochemistry

  1. Tsuji H et al. TDP-43 accelerates age-dependent degeneration of interneurons. Sci Rep. 7, 14972 (2017)(PMID:29097807)
  2. Watanabe S et al. Intracerebroventricular administration of Cystatin C ameliorates disease in SOD1-linked amyotrophic lateral sclerosis mice. J Neurochem. (2017) In press.(PMID:29282717)
Product category
Research area
Autophagy
Ubiquitin proteasome
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  • The availability is based on the information in Japan at 00:05, Sep 20, 2018 in JST.
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  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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