Code No. |
M186-3 |
Anti-LC3 mAb
|
Size |
100 µL (1 mg/mL) |
Availability (in Japan) |
10 or more
(In Japan at 17:00, May 19, 2024 in JST)
|
Clonality |
Monoclonal |
Clone |
8E10 |
Isotype
(Immunized Animal) |
Mouse IgG2aκ |
Applications |
WB 1 µg/mL IP* reported. (PMID: 28924380)
|
Immunogen
(Antigen) |
Human LC3, 1-120 aa Recombinant protein |
Reactivity [Gene ID] |
Human[81631], Mouse[67443], Rat[64862], Hamster[100769810] |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2 |
Storage temp. |
-20°C |
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
microtubule-associated protein 1 light chain 3 beta, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B |
Related products |
PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb PD014 Anti-LC3 pAb PM045 Anti-p62 (SQSTM1) pAb M162-3 Anti-p62 (SQSTM1) (Human) mAb PM066 Anti-p62 C-terminal pAb PM072 Anti-VMP1 pAb PD017 Anti-Beclin 1 pAb PM037 Anti-GABARAP pAb M135-3 Anti-GABARAP mAb PM038 Anti-GATE-16 pAb M133-3 Anti-Atg3 mAb PM039 Anti-Atg7 (Human) pAb 8485 Autophagy Ab Sampler Set PM036-PN Positive control for anti-LC3 antibody PM090 Anti-Atg8 (Filamentous fungi) pAb M152-3MS Anti-LC3 mAb for My select sampler set 8486 Autophagy Watch M134-3 Anti-Atg4B mAb M154-3 Anti-Atg12 (Human) mAb M162-A48 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 488 M162-A59 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 594 M162-A64 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 647 M186-7 Anti-LC3 mAb-HRP-DirecT M200-3 Anti-NRF2 mAb
|
Notes |
Clone 8E10 reacts with LC3B and does not cross-react with LC3A, LC3C, GATE-16, and GABARAP. |
Product category |
Research area:Autophagy |
Data |
|
Citations |
Western Blotting - Margariti A et al. XBP1 mRNA splicing triggers an autophagic response in endothelial cells through BECLIN-1 transcriptional activation. J Biol Chem 288, 859-72 (2013)(PMID:23184933)
- Mukai R et al. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
- Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421)
- Wang M et al. EGFR-mediated chromatin condensation protects KRAS-mutant cancer cells against ionizing radiation. Cancer Res. 74, 2825-34 (2014)(PMID:24648348)
- He R et al. Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11, 740-7 (2015)(PMID:25955014)
- Hasegawa K et al. Promotion of mitochondrial biogenesis by necdin protects neurons against mitochondrial insults. Nat Commun. 7, 10943 (2016)(PMID:26971449)
- Li Z et al. Ammonia Induces Autophagy through Dopamine Receptor D3 and MTOR. PLoS One 11, e0153526 (2016)(PMID:27077655)
- Nakanishi T et al. Relationship between triterpenoid anticancer drug resistance, autophagy, and caspase-1 in adult T-cell leukemia. PeerJ 12, e2026 (2016)(PMID:27190722)
- Boada-Romero E et al. The T300A Crohn's disease risk polymorphism impairs function of the WD40 domain of ATG16L1. Nat Commun. 7, 11821 (2016)(PMID:27273576)
- Sasaki N et al. Chorein interacts with α-tubulin and histone deacetylase 6, and overexpression preserves cell viability during nutrient deprivation in human embryonic kidney 293 cells. FASEB J. 30, 3726-3732 (2016)(PMID:27468757)
- Maueröder C et al. Capability of Neutrophils to Form NETs Is Not Directly Influenced by a CMA-Targeting Peptide. Front Immunol. 8, 16 (2017) (PMID:28191006)
- Zhao Z et al. Curcumin accelerates the repair of sciatic nerve injury in rats through reducing Schwann cells apoptosis and promoting myelinization. Front Immunol. 8, 16 (2017)(PMID:28622711)
- He R et al. Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep. 7, 10532 (2017)(PMID:28874882)
- Sun Y et al. Dietary potassium regulates vascular calcification and arterial stiffness. JCI insight 2, e94920 (2017)(PMID:28978809)
- Wang H et al. Resveratrol attenuates oxidative damage through activating mitophagy in an in vitro model of Alzheimer's disease. Toxicol Lett. 282, 100-108 (2018)(PMID:29097221)
- Wang Y et al. AG-1031 induced autophagic cell death and apoptosis in C6 glioma cells associated with Notch-1 signaling pathway. J Cell Biochem. 119, 5893-5903(2018)(PMID:29575026)
- Li YC et al. Opposite Effects of Two Human ATG10 Isoforms on Replication of a HCV Sub-genomic Replicon Are Mediated via Regulating Autophagy Flux in Zebrafish. Front Cell Infect Microbiol. 8, 109 (2018)(PMID:29670865)
- Ito Y et al. Synergistic effects of HMG-CoA reductase inhibitor and angiotensin II receptor blocker on load-induced heart failure. FEBS Open Bio. 8, 799-816 (2018)(PMID:29670865)
- Taniguchi K et al. Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence. Am J Cancer Res. 8, 650-661 (2018)(PMID:29736310)
- Kozako T et al. Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines. BMC Cancer. 18, 791 (2018)(PMID:30081901)
- Saito T et al. An alternative mitophagy pathway mediated by Rab9 protects the heart against ischemia. J Clin Invest. 129, 802-819 (2019)
(PMID:30511961)
- Slowicka K et al. Physical and functional interaction between A20 and ATG16L1-WD40 domain in the control of intestinal homeostasis. Nat Commun. 10, 1834 (2019)(PMID:31015422)
- Kokubu Y et al. Phenotypic Drug Screening for Dysferlinopathy Using Patient-Derived Induced Pluripotent Stem Cells. Stem Cells Transl Med. 8, 1017-1029(2019)(PMID:31250983)
- Ma YY et al. IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro. Cell Death Dis. 11, 200 (2020)(PMID:32205851)
- Yamamoto YH et al. ERdj8 governs the size of autophagosomes during the formation process. J Cell Biol. 219, e201903127 (2020)(PMID:32492081)
Immunoprecipitation - Meng XH et al. Intracellular Insulin and Impaired Autophagy in a Zebrafish model and a Cell Model of Type 2 diabetes. Int J Biol Sci. 13, 985-995 (2017)(PMID:28924380)
- Serramito-Gómez I et al. Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain. Nat Commun. 11, 5919 (2020)(PMID:33219218)
Immunocytochemistry - Li Z et al. Ammonia Induces Autophagy through Dopamine Receptor D3 and MTOR. PLoS One 11, e0153526 (2016)(PMID:27077655)
- Meng XH et al. Intracellular Insulin and Impaired Autophagy in a Zebrafish model and a Cell Model of Type 2 diabetes. Int J Biol Sci. 13, 985-995 (2017)(PMID:28924380)
- Li W et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther. 9, 9 (2018)(PMID:29335016)
- Yamamoto YH et al. ERdj8 governs the size of autophagosomes during the formation process. J Cell Biol. 219, e201903127 (2020)(PMID:32492081)
Immunofluorescence - Ma YY et al. IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro. Cell Death Dis. 11, 200 (2020)(PMID:32205851)
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