- How do you check the BrU incorporation rate in the cells ?
The BrU incorporation rate can be confirmed by the following methods:
・Flow cytometry using BrU-labeled cells
・RNA-IP, Dot blot, or ELISA using total RNA derived from BrU-labeled cells
Please select the method best suited for your purpose.
- BrU is not efficiently incorporated into the cells.
Please optimize the BrU concentraiton, pulse-time, or chase-time. BrU concentrations of more than 500 μM may affect cell growth and/or other biological processes.
- BrU-labeled RNA cannot be corrected.
Please optimize the amount of input, antibody, or beads.