Proteins function by interacting with various other proteins. To reveal the function of a protein, it is important to identifiy other proteins which interact to the protein.
p62/SQSTM1 directly interacts with LC3, which is localized on autophagosome membrane, and is degradated by autophagic-lysosome pathway. Interactions of p62/SQSTM1 with LC3 was confirmed by pull-down assay using the cell lysate of the HA-LC3 transfectant.
Beclin 1, which is essential for early autophagosome formation, is reported to make complex with various proteins such as UBRAG and Vps34 and regulate the processes of autophagosome/lysosome fusion (Autophagosome maturation) and endocytosis. Using HA, c-Myc, and His-tagged protein purification kits, pull-down assay of Beclin1 and UVRAG is performed
*Samples: HA tagged Beclin1/Myc-His tagged UVRAG transfectant.