Magnosphere™ MS160/Streptavidin & Magnosphere™ MS300/Streptavidin is magnetic particles, streptavidin immobilized on the hydrophilic surface. Since the non-specific adsorption of such protein, nucleic acid to the particle surface is kept low, high purity biotin-labeled molecules can be recovered from the sample.
The particle surface is coated with a hydrophilic polymer which does not inhibit the enzymatic reaction. Consequently Magnosphere™ MS300/Streptavidin can be added to the PCR, quantitative PCR reaction buffer.
Also this particle can be used as binding carrier of biotinylated antibody for chemiluminescence enzyme immunoassay.
● High affinity to biotinylated proteins or nucleotides
● Uniform particle size
● Rapid magnetic response
● Low non-specific binding
|Particle size||1.5 µm||3 µm|
|Biotin binding capacity||600≦（pmol/mg）||400≦（pmol/mg）|
|Application||Biotinylated ligand coupling
（Immunoassay, DNA/RNA capture, Enzyme immobilization, etc）
Quantitative PCR was performed with Magnosphere™ MS300/Streptavidin added to a known concentration of 100b.p.DNA, and the Threshold Cycle (Ct) was plotted. Ct was constant with no PCR inhibition up to 3 mg of Magnosphere™ MS300/Streptavidin added to the reaction.
An EIA test was performed by binding a biotinylated anti-AFP antibody to Magnosphere™ MS300/Streptavidin and the competitor’s avidin particle. The signal intensity was measured by varying the AFP concentration. Magnosphere™ MS300/Streptavidin showed a higher signal level than the competitor's particle.
|Biotine binding capacity (pmol/mg)||Particle Contents (%)||Volume
Magnosphere is a trademark of JSR corporation.