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Anti-Phospho-p62 antibody

Hot topic in research on neurodegenerative disease and cancer

p62 contains multiple phosphorylation sites. Sequential phosphorylation of these sites regulates biological defense mechanisms such as selective autophagy.
The phosphorylation of Ser407 (human)/Ser409 (mouse) precedes the phosphorylation of Ser403 (human)/Ser405 (mouse) in p62, which increases its affinity for poly ubiquitin chains. Consequently, ubiquitinated abnormal protein aggregates, depolarized mitochondria, and invading intracellular bacteria are sequestered by phospho-p62. Further phosphorylation of Ser349 (human)/Ser351 (mouse) by mTORC1 increases the affinity of p62 for Keap1, inducing dissociation of Nrf2 from Keap1 and nuclear translocation of Nrf2 (the p62-Keap1-Nrf2 pathway). Nrf2 is a stress-response transcription factor and activates the transcription of various stress resistance genes. Nrf2 also induces p62 gene expression, forming a positive feedback loop. Phospho-p62 with bound Keap1 interacts with LC3 through the LIR (LC3-interacting region) and is degraded by the autophagy pathway. Thus, the cells under stress conditions effectively overcome their negative environment by activating two biological defense mechanisms through the phosphorylation of p62.
Impaired selective autophagy is implicated in various diseases. For example, neurons in familial parkinsonism fail to clear protein aggregates and depolarized mitochondria, resulting in neuronal damage and compromised brain function. In hepatocarcinoma cells, p62 is constitutively phosphorylated at Ser349, causing continuous activation of Nrf2. Hence, inhibitors of p62 phosphorylation and inhibitors of the interaction between phospho-p62 and Keap1 have the potential to be novel cancer therapeutics.
(Reference: Saito, T., et al., Nat. Commun. 7, 12030 (2016) PMID: 27345495).

■ p62-Keap1-Nrf2 Pathway
Click to enlarge

Phosphorylated p62 related antibodies

  Isotype (Immunized Animal)
Mouse Rabbit Rat
Multi Ubiquitin

Product data; Anti-Phospho-p62 antibodies

■ Western blotting
Phospho-p62 (Ser403)(Code No. D343-3, Clone:4F6)Western blotting

■ Immunohistochemistry
Phospho-p62 (Ser403)(Code No. D343-3, Clone:4F6)Immunocytochemistry

■ Immunohistochemistry
Phospho-p62 (Ser403)(Code No. D344-3, Clone: 4C8)Immunohistochemistry

■ Western blotting
Anti-Phospho-p62 (SQSTM1) (Ser351) mAb(Code No. M217-3)Western blotting

■ Immunohistochemistry
Anti-Phospho-p62 (SQSTM1) (Ser351) mAb(Code No. M217-3)Immunocytochemistry

■ Western blotting
Anti-Phospho-p62 (SQSTM1) (Ser351) pAbCode No. PM074)Western blotting

■ Immunocytochemistry
Anti-Phospho-p62 (SQSTM1) (Ser351) pAbCode No. PM074)Immunocytochemistry

Product data; Anti-NRF2 antibodies

Anti-NRF2 mAb(Code No. M200-3, Clone: 1F2)

Anti-NRF2 pAb(Code No. PM069)製品データ

Product data; Anti-KEAP1 antibody

  • High sensitivity, low background

Anti-KEAP1 mAb(Code No. M224-3)製品データ

Product data; Anti-Ubiquitin antibodies

  • Mono ubiquitin Specific

Western Blotting (MK-11-3)

Western Blotting (MK-12-3)

Product data; Multi-Ubiquitin antibodies

  • React to malti ubiquitin and mono ubiquitin

Western Blotting (D058-3)

  • Multi ubiquitin Specific

Western Blotting (D071-3)