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QuickSwitch™ Custom Tetramer Kits
Kits for preparation of custom tetramers in the laboratory using our proprietary peptide exchange technology.
Features
- Prepare custom tetramers in 4 hours
- No UV lamp or special instrument required
- Quantify peptide exchange efficiency (Quant Tetramer Kits)
- Select ready-to-use tetramer in PE, APC, or BV421
Principle of the peptide exchange reaction
MHC tetramers in QuickSwitch™ Custom Tetramer Kits are pre-bound with "exiting peptide" (shown in blue) to maintain structural integrity. Exchange of the exiting peptide with custom peptide (shown in red) is initiated upon addition of the custom peptide and a peptide-exchange factor (reaction time is 4 hours).
The efficiency of peptide exchange depends on the sequence of the custom peptide. QuickSwitch™ Quant Tetramer Kits contain reagents for determination of the peptide exchange efficiency.
Workflow
Peptide exchange, quantification, cell staining, and flow cytometry analysis can all be performed in one day.
Determination of peptide exchange efficiency
QuickSwitch™ Quant Tetramer Kits contain FITC labeled antibody which detects to the exiting peptide pre-bound to MHC molecules. After the peptide-exchange reaction, the MHC tetramers are adsorbed to special beads and reacted with the antibody for the determination of peptide exchange efficiency by flow cytometry.
