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HOME > Product search results > Code No. TB-7400-K2 QuickSwitch™ Quant H-2Kb Tetramer Kit-APC

Code No. TB-7400-K2

QuickSwitch™ Quant H-2Kb Tetramer Kit-APC

Price

¥157,000

Availability (in Japan)

0
(Contact us)

(In Japan at 00:05,
Apr 17, 2024 in JST)

Size

25 µg

The QuickSwitch™ Quant Tetramer Kit utilizes a patented technique for exchanging up to ten peptides on an MHC class I tetramer. Components for quantifying the extent of peptide exchange by flow cytometry are included. New specificity tetramers obtained by peptide exchange can then be used for identification of antigen-specific CD8+ T lymphocytes in staining assays.
Data
  • %Peptide Exchange of TRP2 (SVYDFFVWV)  and OVA (SIINFEKL).

    %Peptide Exchange of TRP2 (SVYDFFVWV)  and OVA (SIINFEKL).

  • H2-Kb Peptide-exchanged tetramers perform similarly to classically folded tetramers.

    H2-Kb Peptide-exchanged tetramers perform similarly to classically folded tetramers.
    H-2 Kb TRP2 used a negative control (green), classically folded H-2 Kb OVA (blue), and H-2 Kb QuickSwitch™ OVA (red) tetramer staining.

Class Class I Animal species Mouse
Category Others
Conjugate APC
Immunogen (Antigen) user's selected
Sequence -
MHC Allele H-2Kb
Location (aa) - Storage temp. 4°C Manufacturer MBL International Corporation
Related products TB-7400-K1 QuickSwitch™ Quant H-2Kb Tetramer Kit-PE
TB-7400-K4 QuickSwitch™ Quant H-2Kb Tetramer Kit-BV421
Note (Kit Component)
QuickSwitch™ Tetramer: (500 μL x 1 amber vial, 25 μg)
Peptide Exchange Factor: (13 μL x 1 clear vial)
Magnetic Capture Beads: (500 μL x 1 clear vial)
Exiting Peptide Antibody-FITC (25x) (25 μL x 1 amber vial)
Reference Peptide: (1 mM)
Assay Buffer (10x): (1.7 mL x 1 vial)
Citations

Flow Cytometry

  1. Song X et al. Targeting cluster of differentiation 47 improves the efficacy of anti-cytotoxic T-lymphocyte associated protein 4 treatment via antigen presentation enhancement in pancreatic ductal adenocarcinoma. Exp Ther Med. 20, 3301-3309 (2020)(PMID:32855701)
Product category
Research area
Immunology
Tools
MHC tetramer
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