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HOME > Product search results > Code No. CY-1253V2 CycLex® NAD+/NADH Colorimetric Assay Kit Ver.2

Code No. CY-1253V2

CycLex® NAD+/NADH Colorimetric Assay Kit Ver.2

Price

¥52,000

Availability (in Japan)

1-9

(In Japan at 17:00,
Nov 22, 2019 in JST)

Size

100 Assays

Data
  • Experimental example

    Experimental example
    NAD+ and NADH concentrations in cell extracts of SW480 and Jurkat cells.

  • Effect of FK866 on NAD+ and NADH concentrations

    Effect of FK866 on NAD<sup>+</sup> and NADH concentrations
    NAD+ and NADH concentrations in cell extracts of Jurkat cells treated with NAMPT specific inhibitor FK866 at indicated concentrations.

  • Time course of NADH standards

    Time course of NADH standards

Components NAD+/NADH Assay Buffer (20X), WST-1, ADH (alcohol dehydrogenase), Diaphorase, EtOH Solution, 400 µM NADH, 20X Standard Dilution Buffer
Intended use This Kit provides a convenient method for sensitive measurement of NAD+, NADH and their ratio in cell extracts. This kit is designed for specific detection of NAD+ and NADH by an enzyme cycling reaction.
Storage temp. -70°C Manufacturer MBL
Sample Cell lysate
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References
  1. Miki A et al. Loss of aquaporin 9 expression adversely affects the survival of retinal ganglion cells. Am J Pathol. 182, 1727-39 (2013)(PMID: 23506846
  2. Adamovich Y et al. The protein level of PGC-1α, a key metabolic regulator, is controlled by NADH-NQO1. Mol Cell Biol. 33, 2603-13 (2013)(PMID: 23648480
  3. Ji B et al. Inhibition of protein translation by the DISC1-Boymaw fusion gene from a Scottish family with major psychiatric disorders. Hum Mol Genet. 23, 5683-705 (2014)(PMID: 24908665
  4. Prud'homme GJ et al. GABA protects pancreatic beta cells against apoptosis by increasing SIRT1 expression and activity. Biochem Biophys Res Commun. 452, 649-54 (2014)(PMID: 25193706
Product category
Research area
Cancer
Drug discovery
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