This site is for customers in Japan. Customers in other regions, please go to Global page.
HOME > Product search results > Code No. CY-1160 CycLex® Rho-kinase Assay Kit

Code No. CY-1160

CycLex® Rho-kinase Assay Kit



Availability (in Japan)

(Contact us)

(In Japan at 17:00,
Sep 28, 2021 in JST)


96 assays


Components Microplate (coated with recombinant MBS C-terminus(758-1032 a.a.) as Rho-kinase substrate), 10×Wash Buffer, Kinase Buffer, 20×ATP, HRP conjugated Detection Antibody, Substrate Reagent, Stop Solution
Intended use This Kit is primarily designed to measure the activities of purified Rho-kinase or DMPK for the rapid and sensitive evaluation of inhibitors or activators.
Storage temp. 4°C Manufacturer MBL
Related products CY-M1011 Anti-Phospho-MBS (MYPT1) (Thr696) mAb
  1. Massaro M et al. Statins inhibit cyclooxygenase-2 and matrix metalloproteinase-9 in human endothelial cells: anti-angiogenic actions possibly contributing to plaque stability. Cardiovasc Res. 86, 311-20 (2010)(PMID:19946014)
  1. Xu Y et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. PNAS. 107, 8129-34 (2010)(PMID:20406903)
  1. Torre C et al. Reduction of Hyaluronan-CD44-Mediated Growth, Migration, and Cisplatin Resistance in Head and Neck Cancer Due to Inhibition of Rho Kinase and PI-3 Kinase Signaling. Arch Otolaryngol Head Neck Surg. 136, 493-501 (2010)(PMID:20479382)
  1. Lapointe TK et al. Interleukin-1 receptor phosphorylation activates Rho kinase to disrupt human gastric tight junctional claudin-4 during Helicobacter pylori infection. Cellular Microbiology 12, 692-703 (2010)(PMID:20070312)
  1. Bivalacqua TJ et al. Attenuated RhoA/Rho-kinase Signaling in Penis of Transgenic Sickle Cell Mice. Urology 76, 510.e7-12 (2010)(PMID:20538321)
  1. Liu S et al. Polychlorinated Biphenyls (PCBs) Enhance Metastatic Properties of Breast Cancer Cells by Activating Rho-Associated Kinase (ROCK). PLoS One 5, e11272 (2010)(PMID:20585605)
  1. Géraud C et al. Liver sinusoidal endothelium: A microenvironment-dependent differentiation program in rat including the novel junctional protein liver endothelial differentiation-associated protein-1. Hepatology 52, 313-26 (2010)(PMID:20578158)
  1. Fujimura M et al. Inhibition of the Rho/ROCK pathway prevents neuronal degeneration in vitro and in vivo following methylmercury exposure. Toxicol Appl Pharmacol. 250, 1-9 (2011)(PMID:20869980)
  1. Duong-Quy S et al. Increased Rho-kinases expression and activity and pulmonary endothelial dysfunction in smokers with normal lung function. Eur Respir J. 37, 349-55 (2011)(PMID:20530044)
  1. Smitasiri Y et al. An Initial Evaluation of the Safety, Efficacy and Purity of VigRX, a Herbal Combination Formula, for the Enhancement of Male Sexual Health. The Open Natural Products Journal 3, 10-19 (2010)
  1. Gu X, Masters KS. Role of the Rho pathway in regulating valvular interstitial cell phenotype and nodule formation. Am J Physiol Heart Circ Physiol. 300, H448-58 (2011)(PMID:21131478)
  1. Kirabo A et al. Vascular smooth muscle Jak2 mediates angiotensin II-induced hypertension via increased levels of reactive oxygen species. Cardiovasc Res. 91, 171-9 (2011)(PMID:21354995)
  1. Riganti C et al. Atorvastatin modulates anti-proliferative and pro-proliferative signals in Her2/neu-positive mammary cancer. Biochem Pharmacol. 82, 1079-89 (2011)(PMID:21803026)
  1. Haidari M et al. Inhibition of MLC phosphorylation restricts replication of influenza virus--a mechanism of action for anti-influenza agents. PLoS One 6, e21444 (2011)(PMID:21731751)
  1. Nölle A et al. The Spinal Muscular Atrophy disease protein SMN is linked to the Rho-kinase pathway via profilin. Hum Mol Genet. 20, 4865-78 (2011)(PMID:21920940)
  1. Bagi Z et al. Increased availability of angiotensin AT 1 receptors leads to sustained arterial constriction to angiotensin II in diabetes - role for Rho-kinase activation. Br J Pharmacol. 163, 1059-68 (2011)(PMID:21385178)
  1. Kobori H et al. Rho-kinase/nuclear factor-κβ/angiotensinogen axis in angiotensin II-induced renal injury. Hypertens Res. 34, 976-9 (2011)(PMID:21633359)
  1. Shahin R et al. Elaborate ligand-based modeling reveal new submicromolar Rho kinase inhibitors. J Comput Aided Mol Des. 26, 249-66 (2012)(PMID:22167443)
  1. Morckel AR et al. A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos. Development 139, 437-42 (2012)(PMID:22186732)
  1. Luke T et al. Kinase dependent activation of voltage-gated Ca2+ channels by ET-1 in pulmonary arterial myocytes during chronic hypoxia. Am J Physiol Lung Cell Mol Physiol. 302, L1128-39 (2012)(PMID:22387294)
  1. Chang TC et al. Rho Kinases Regulate the Renewal and Neural Differentiation of Embryonic Stem Cells in a Cell Plating Density-Dependent Manner. PLoS One. 5, e9187 (2010)(PMID:20169147)
  1. Naraoka M et al. Suppression of the Rho/Rho-kinase pathway and prevention of cerebral vasospasm by combination treatment with statin and fasudil after subarachnoid hemorrhage in rabbit. Transl Stroke Res. 4, 368-74 (2013)(PMID:23658597)
  1. Dush MK, Nascone-Yoder NM. Jun N-terminal kinase maintains tissue integrity during cell rearrangement in the gut. Development. 140, 1457-66 (2013)(PMID:23462475)
  1. Liang JL et al. Effects of interleukin-1β on vascular reactivity after lipopolysaccharide-induced endotoxic shock in rabbits and its relationship with PKC and Rho kinase. J Cardiovasc Pharmacol. 62, 84-9 (2013)(PMID:23846803)
  1. Riganti C et al. Zoledronic acid restores doxorubicin chemosensitivity and immunogenic cell death in multidrug-resistant human cancer cells. PLoS One. 8, e60975 (2013)(PMID:23593363)
  1. Takata M et al. Fasudil, a rho kinase inhibitor, limits motor neuron loss in experimental models of amyotrophic lateral sclerosis. Br J Pharmacol. 170, 341-51 (2013)(PMID:23763343)
  1. Bourguignon LY et al. Selective matrix (hyaluronan) interaction with CD44 and RhoGTPase signaling promotes keratinocyte functions and overcomes age-related epidermal dysfunction. J Dermatol Sci. 72, 32-44 (2013)(PMID:23790635)
  1. Bei Y et al. Activation of RhoA/Rho-kinase pathway accounts for pulmonary endothelial dysfunction in patients with chronic obstructive pulmonary disease. Physiol Rep. 1, e00105 (2013)(PMID:24303177)
  1. Sari AN et al. Contribution of RhoA/Rho-kinase/MEK1/ERK1/2/iNOS pathway to ischemia/reperfusion-induced oxidative/nitrosative stress and inflammation leading to distant and target organ injury in rats. Eur J Pharmacol. 723, 234-45 (2014)(PMID:24296316)
  1. Kheirollah A et al. Involvement of cdc42/Rho kinase in apoA-I-mediated cholesterol efflux through interaction between cytosolic lipid-protein particles and microtubules in rat astrocytes. J Neurosci Res. 92, 455-63 (2014)(PMID:24446142)
  1. Li YH et al. Intranasal delivery of FSD-C10, a novel Rho kinase inhibitor, exhibits therapeutic potential in experimental autoimmune encephalomyelitis. Immunology. 143, 219-29 (2014)(PMID:24749492)
  1. Liu D et al. The glucocorticoid dexamethasone inhibits U937 cell adhesion and neutrophil release via RhoA/ROCK1-dependent and independent pathways. Cell Physiol Biochem. 33, 1654-62 (2014)(PMID:24902944)
  1. Pinzón-Daza ML et al. The cross-talk between canonical and non-canonical Wnt-dependent pathways regulates P-glycoprotein expression in human blood-brain barrier cells. J Cereb Blood Flow Metab. 34, 1258-69 (2014)(PMID:24896565)
  1. Chen Z et al. cIMP synthesized by sGC as a mediator of hypoxic contraction of coronary arteries. Am J Physiol Heart Circ Physiol. 307, H328-36 (2014)(PMID:24906916)
  1. Kwok W and Clemens MG. Rho-kinase activation contributes to Lps-induced impairment of endothelial nitric oxide synthase activation by endothelin-1 in cultured hepatic sinusoidal endothelial cells. Shock. 42, 554-61 (2014)(PMID:25243430)
  1. Li H et al. Design, synthesis and evaluation of biological activity of novel fasudil analogues. Pharmazie. 69, 867-73 (2014)(PMID:25951658)
  1. Rigoni M et al. Simvastatin and downstream inhibitors circumvent constitutive and stromal cell-induced resistance to doxorubicin in IGHV unmutated CLL cells. Oncotarget 6, 29833-46 (2015)(PMID:26284584)
  1. Gagliano-Jucá T et al. Hydrochlorothiazide Potentiates Contractile Activity of Mouse Cavernosal Smooth Muscle. Sex Med. 4, e113-23 (2016)(PMID:27006319)
  1. Hong J et al. Rho Kinase ROCK2 Mediates Acid-Induced NADPH Oxidase NOX5-S Expression in Human Esophageal Adenocarcinoma Cells. PLoS One. 11, e0149735 (2016)(PMID:26901778)
  1. Yuan TY et al. DL0805-2, a novel indazole derivative, relaxes angiotensin II-induced contractions of rat aortic rings by inhibiting Rho kinase and calcium fluxes. Acta Pharmacol Sin. 37, 604-16 (2016)(PMID:27041459)
  1. Hannan JL et al. Caspase-3 dependent nitrergic neuronal apoptosis following cavernous nerve injury is mediated via RhoA and ROCK activation in major pelvic ganglion. Sci Rep. 6, 29416(2016)(PMID:27388816)
  1. Zhang L et al. Dimethyloxalylglycine Promotes Bone Marrow Mesenchymal Stem Cell Osteogenesis via Rho/ROCK Signaling. Cell Physiol Biochem. 39, 1391-403 (2016)(PMID:27606625)
  1. Takaoka M et al. FKBP51 regulates cell motility and invasion via RhoA signaling. Cancer Sci. 108, 380-389 (2017)(PMID:28032931)
  1. Yao Y et al. Discovery of Novel N-Substituted Prolinamido Indazoles as Potent Rho Kinase Inhibitors and Vasorelaxation Agents. Molecules 22, E1766 (2017)(PMID:29048389)
  1. Kashiwagi K et al. A Ser75-to-Asp phospho-mimicking mutation in Src accelerates ageing-related loss of retinal ganglion cells in mice. Sci Rep. 7, 16779 (2017)(PMID:29196663)
  1. Huang L et al. The influence of human TDP-43-M337V on ROCK/PTEN signaling pathway in NSC-34 cells. Int J Clin Exp Med. 11, 3596-3603 (2018)
  1. Wang X et al. FSD-C10 Shows Therapeutic Effects in Suppressing oxidized low-density lipoprotein (ox-LDL)-Induced Human Brain Microvascular Endothelial Cells Apoptosis via Rho-Associated Coiled-Coil Kinase (ROCK)/Mitogen-Activated Protein Kinase (MAPK) Signaling. Med Sci Monit. 24, 5509-5516 (2018)(PMID:30088495)
  1. Li H et al. Design, Synthesis, and Biological Evaluations of Several Fasudil Analogues. J Het Chem. 56, 1 (2018)
  1. Kato G. Nonphosphorylatable Src Ser75 Mutation Increases Ethanol Preference and Consumption in Mice. eNeuro. 6, pii: ENEURO.0418-18.2019. doi: 10.1523/ENEURO.0418-18.2019. (2019) (PMID:30963106)
  1. Milosevic V et al. Wnt/IL-1β/IL-8 autocrine circuitries control chemoresistance in mesothelioma initiating cells by inducing ABCB5. Int J Cancer. 146, 192-207 (2020)(PMID:31107974)
Product category
Research area
Drug discovery
Signal transduction

  • The availability is based on the information in Japan at 17:00, Sep 28, 2021 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.