Antibody detailed product information

Code No. RN033P
Anti-TNRC6A (GW182) (Human) pAb
Price ¥66,000
Size 200 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 18, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit Ig (aff.)
Applications WB　1 µg/mL  
IP　5 µg/500 µL of cell extract from 1 x 107 cells  
IC　reported  （PMID: 21935400 / 26990993）
RIP　15 µg/500 µL of cell extract from 1.3 x 107 cells  
Other(eCLIP)　reported.  （PMID: 27018577）
Immunogen
(Antigen)		 KLH conjugated synthetic peptide, corresponding to internal region of human TNRC6A
Reactivity [Gene ID]

Human[27327], Mouse(-), Rat(-)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names GW1, TNRC6, CAGH26
Background The trinucleotide repeat containing 6A (TNRC6A), also known as GW182, was so named because of its molecular weight and the presence of multiple glycine (G)–tryptophan (W) amino acid pairs in the N-terminal region that is required for interaction with Argonaute proteins. GW182 is a critical component of cytoplasmic processing bodies (P-bodies), which have been shown to function in mRNA degradation, storage, and miRNA-mediated gene silencing. The interaction of GW182 with Argonautes is required for P-body localization and may be critical for miRNA-mediated translational repression, because it induces the formation of the complexes containing miRNA–target mRNA. Further, the phosphorylated form of human TNRC6A has been shown to play a role in miRNA mediated gene silencing.
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Product category Research area:RNA-RNP Network Exosomes
Brands:RiboCluster Profiler™
Data



Citations

Western Blotting

  1. Otsuka M et al. Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma. PLoS One. 6, e24359 (2011)(PMID:21935400)
  2. Fukao A et al. MicroRNAs Trigger Dissociation of eIF4AI and eIF4AII from Target mRNAs in Humans. Mol Cell 56, 79-89 (2014)(PMID:25280105)
  3. Yamagishi M et al. Coordinated loss of microRNA group causes defenseless signaling in malignant lymphoma. Sci Rep. 5, 17868 (2015)(PMID:26639163)
  4. Kami D et al. The DEAD-box RNA-binding protein DDX6 regulates parental RNA decay for cellular reprogramming to pluripotency. PLoS One. 13, e0203708 (2018)(PMID:30273347)

Immunocytochemistry

  1. Otsuka M et al. Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma. PLoS One. 6, e24359 (2011)(PMID:21935400)
  2. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)

RNP Immunoprecipitation

  1. Yamagishi M et al. Coordinated loss of microRNA group causes defenseless signaling in malignant lymphoma. Sci Rep. 5, 17868 (2015)(PMID:26639163)

Other(eCLIP)

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
  • The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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