Antibody detailed product information

Code No. RN017M
Anti-7-methylguanosine (m7G) mAb
This antibody (clone 4141-13) reacts with both 5′-terminal and internal 7-methylguanosine (m7G) in RNA.
Price ¥66,000
Size 200 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 20, 2024 in JST）
Clonality Monoclonal
Clone 4141-13
Isotype
(Immunized Animal) 		Mouse IgG2aκ
Applications IP　10 µg/sample  
IC　Can be used.  
ELISA　Can be used.  
DB　1 µg/mL  
Other(Northwestern blotting)　reported.  （PMID: 29983320）
Immunogen
(Antigen)		 Carrier protein-conjugated 7-methylguanosine (m7G)-Cap analogue
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Background The 7-methylguanosine (m7G) is a modified nucleoside commonly found in eukaryotes. The m7G modification present on the 5′-end of mRNA and on some ncRNA synthesized by RNA polymerase II is called m7G cap. It plays a role in transcription, splicing, polyadenylation, nuclear export, stabilization, and translation. In addition, other m7G modifications are also found in the tRNA or rRNA. The m7G46 modification occurs in bacterial and eukaryotic tRNAs. The m7G1575 modification in the 18S rRNA is evolutionarily conserved in eukaryotes.
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Product category Research area:RNA-RNP Network Epigenetics
Brands:RiboCluster Profiler™
Data



Citations

Immunoprecipitation

  1. Lin S et al. Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Mol Cell 71, 244-255.e5 (2018)(PMID:29983320)

RNP Immunoprecipitation

  1. Pandolfini L et al. METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation. Mol Cell. pii: S1097-2765(19)30269-2 (2019)(PMID:31031083)

Other(Northwestern blotting)

  1. Lin S et al. Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Mol Cell 71, 244-255.e5 (2018)(PMID:29983320)
References
  1. Maden BE, Salim M. The methylated nucleotide sequences in HELA cell ribosomal RNA and its precursors. J Mol Biol. 88, 133-52 (1974)（PMID: 4374549 ）
  2. Cowling VH. Regulation of mRNA cap methylation. Biochem J. 425, 295-302 (2009)（PMID: 20025612）
  3. Cubrilo S et al. The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site. RNA 15, 1492-7 (2009)（PMID: 19509304）
  4. Chen P et al. Transfer RNA modifications and genes for modifying enzymes in Arabidopsis thaliana. BMC Plant Biol. 10, 201 (2010)（PMID: 20836892）
  5. Filonava L et al. A novel cause for primordial dwarfism revealed: defective tRNA modification. Genome Biol. 16, 216 (2015)（PMID: 26429597 ）
  6. Zorbas C et al. The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis. Mol Biol Cell. 26, 2080-95 (2015)（PMID: 25851604 ）
  • The availability is based on the information in Japan at 17:00, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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