Code No. | PM021 | |
Anti-S-tag pAb | ||
Price | ¥35,200 | |
Size | 100 µL | |
Availability (in Japan) | 1-9
(In Japan at 17:00, Apr 26, 2024 in JST) |
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Clonality | Polyclonal | |
Clone | Polyclonal | |
Isotype (Immunized Animal) |
Rabbit Ig (aff.) | |
Applications | WB 1:1,000 IP 5 µL/sample |
|
Immunogen (Antigen) |
Carrier protein (CP) conjugated synthetic peptide (CP-KETAAAKFERQHIDSC) | |
Storage buffer | PBS/50% glycerol, pH 7.2 | |
Storage temp. | -20°C | |
Conjugate | Unlabeled | |
Manufacturer | MBL | |
Background | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-tag is an epitope tag composed of a 15 residues peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. S-tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-tag fusion proteins by enzymatic assays. However, antibody against the S-tag sequence provides a more convenient and flexible method to easily identify, detect, or purify S-tag containing fusion proteins. |
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Product category | Tools:Epitope tags | |
Data | ||
Citations | Western Blotting
Immunoprecipitation
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