Antibody detailed product information

Code No. PM005MS
Anti-RFP pAb for My select sampler set
The antibody reacts with DsRed, mRFP1, mCherry, mOrange, mPlum, mStrawberry and tdTomato
Price ¥11,000
Size 20 µL
Availability (in Japan) 1-9
(In Japan at 00:05, Apr 19, 2024 in JST)
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal)
Rabbit IgG
Applications WB 1:1,000  
IC 1:500  
IH* reported.  (PMID: 21331036 / 20534674 / 20008127)
Other(Immuno-EM) reported.  (PMID: 29481606)
Immunogen
(Antigen)
RFP
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names Red fluorescent protein
Background Expression vectors containing a protein and a tag protein are commonly used. Red Fluorescent Protein (RFP) fusion protein expression system is preferably used in various laboratories because of an easy monitoring of fusion proteins. This specific antibody for RFP is useful tool for monitoring of the fusion protein expression.
Related products M155-3 Anti-RFP mAb
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M165-11 Anti-RFP mAb-Magnetic Beads
M204-7 Anti-RFP mAb HRP-DirecT
M208-3 Anti-RFP mAb Cocktail
M204-3 Anti-RFP mAb
PM005 Anti-RFP pAb
PM005-7 Anti-RFP pAb-HRP-DirecT
Product category Tools:Epitope tags Fluorescent proteins
Data


Citations

Western Blotting

  1. Ishida Y et al. Autophagic elimination of misfolded procollagen aggregates in the endoplasmic reticulum as a means of cell protection. Mol Biol Cell. 20, 2744-54 (2009)(PMID:19357194)
  2. Yamamoto H et al. Functional cross-talk between Rab14 and Rab4 through a dual effector, RUFY1/Rabip4. Mol Biol Cell. 21, 2746-55 (2010)(PMID:20534812)
  3. Valbuena N et al. The Vam6 and Gtr1-Gtr2 pathway activates TORC1 in response to amino acids in fission yeast. J Cell Sci. 125, 1920-8 (2012)(PMID:22344254)
  4. Funabashi T et al. Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol Biol Cell. 28, 624-63 (2017)(PMID:28077622)
  5. Satoh R et al. Distinct modes of stress granule assembly mediated by the KH-type RNA-binding protein Rnc1. Genes Cells. 23, 778-785 (2018)(PMID:30014536)
  6. Hirashima T et al. The ABD on the nascent polypeptide and PH domain are required for the precise Anillin localization in Drosophila syncytial blastoderm. Sci Rep. 8, 12910 (2018)(PMID:30150713)
  7. Purvanov V et al. Fluorescently Tagged CCL19 and CCL21 to Monitor CCR7 and ACKR4 Functions. Int J Mol Sci. 19, pii: E3876 (2018) (PMID:30518137)
  8. Sugasawa T et al. Detection of Transgenes in Gene Delivery Model Mice by Adenoviral Vector Using ddPCR. Genes (Basel). 10, pii: E436 (2019)(PMID:31181711)
  9. Murphy JT et al. Simple nutrients bypass the requirement for HLH-30 in coupling lysosomal nutrient sensing to survival. PLoS Biol. 17, e3000245 (2019)(PMID:31086360)
  10. Matsuda T, Oinuma I Optimized CRISPR/Cas9-mediated in vivo genome engineering applicable to monitoring dynamics of endogenous proteins in the mouse neural tissues. Sci Rep. 9, 11309 (2019)(PMID:31383899)
  11. Takano S et al. A novel system of bacterial cell division arrest implicated in horizontal transmission of an integrative and conjugative element. PLoS Genet. 15, e1008445 (2019)(PMID:31069967)

Immunocytochemistry

  1. Kennedy MJ et al. Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods. 7, 973-5 (2010)(PMID:21037589)
  2. Arimoto-Matsuzaki K et al. TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nat Commun. 7, 10252 (2016)(PMID:26738979)

Immunohistochemistry

  1. Humphreys BD et al. Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis. Am J Pathol. 176, 85-97 (2010)(PMID:20008127)
  2. Skora AD, Spradling AC. Epigenetic stability increases extensively during Drosophila follicle stem cell differentiation. PNAS 107, 7389-94 (2010)(PMID:20368445)
  3. Suzuki M et al. MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization. Development. 137, 2329-39 (2010)(PMID:20534674)
  4. Okumura T et al. Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila. Dev Biol. 344, 693-706 (2010)(PMID:20553709)
  5. Williams SE et al. Asymmetric cell divisions promote Notch-dependent epidermal differentiation. Nature. 470, 353-8 (2011)(PMID:21331036)
  6. Letzkus JJ et al. A disinhibitory microcircuit for associative fear learning in the auditory cortex. Nature 480, 331-5 (2011)(PMID:22158104)
  7. Nakazawa N et al. A novel Cre/loxP system for mosaic gene expression in the Drosophila embryo. Dev Dyn. 241, 965-74 (2012)(PMID:22437963)
  8. Daadi MM et al. Imaging neural stem cell graft-induced structural repair in stroke. Cell Transplant. 22, 881-92 (2013)(PMID:23044338)
  9. Chen AE et al. Functional evaluation of ES cell-derived endodermal populations reveals differences between Nodal and Activin A-guided differentiation. Development. 140, 675-86 (2013)(PMID:23293299)
  10. Nagao M et al. Zbtb20 promotes astrocytogenesis during neocortical development. Nat Commun. 7, 11102 (2016)(PMID:27064487)
  11. Wang N et al. Regulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status. Stem Cell Reports. 6, 668-678 (2016)(PMID:27167157)
  12. Tuschl K et al. Mutations in SLC39A14 disrupt manganese homeostasis and cause childhood-onset parkinsonism-dystonia. Nat Commun. 7, 11601 (2016)(PMID:27231142)
  13. Spiró Z et al. Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep. 6, 27470 (2016)(PMID:27273160)
  14. Fujii M et al. Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle. Nat Commun. 8, 14664 (2017)(PMID:28287088)
  15. Woodard LE et al. Kidney-specific transposon-mediated gene transfer in vivo. Sci Rep. 7, 44904 (2017)(PMID:28317878)
  16. Shainer I et al. Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish. Sci Rep. 7, 44777 (2017)(PMID:28317906)
  17. Hoerder-Suabedissen A et al. Subset of Cortical Layer 6b Neurons Selectively Innervates Higher Order Thalamic Nuclei in Mice. Cereb Cortex. 28, 1882-1897 (2018)(PMID:29481606)
  18. Iwasaki K et al. Ablation of Central Serotonergic Neurons Decreased REM Sleep and Attenuated Arousal Response. Front Neurosci. 12, 535 (2018)(PMID:30131671)
  19. Hirashima T et al. The ABD on the nascent polypeptide and PH domain are required for the precise Anillin localization in Drosophila syncytial blastoderm. Sci Rep. 8, 12910 (2018)(PMID:30150713)
  20. Du XF et al. A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis. Sci Rep. 8, 14077 (2018)(PMID:30232367)
  21. Bin Saifullah MA et al. Cell type-specific activation of mitogen-activated protein kinase in D1 receptor-expressing neurons of the nucleus accumbens potentiates stimulus-reward learning in mice. Sci Rep. 8, 14413 (2018)(PMID:30258218)
  22. Plein A et al. Erythro-myeloid progenitors contribute endothelial cells to blood vessels. Nature 562, 223-228 (2018)(PMID:30258231)
  23. Allahyari RV et al. Sonic hedgehog signaling is negatively regulated in reactive astrocytes after forebrain stab injury. Sci Rep. 9, 565 (2019) (PMID:30679745)
  24. Cagnet S et al. Oestrogen receptor α AF-1 and AF-2 domains have cell population-specific functions in the mammary epithelium.(PMID:30413705)

Other(Immuno-EM)

  1. Hoerder-Suabedissen A et al. Subset of Cortical Layer 6b Neurons Selectively Innervates Higher Order Thalamic Nuclei in Mice. Cereb Cortex. 28, 1882-1897 (2018)(PMID:29481606)
  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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