Antibody detailed product information

Code No. PD010
Anti-ATBF1 (D1-120) pAb
Price ¥52,800
Size 100 µL
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 23, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit IgG
Applications WB　1:2,000  
IP　1 µL/150 µg of cell lysate  
IC　1：500  
IH　1:2,500-1:5,000  
IF　1:500  
Immunogen
(Antigen)		 recombinant region of human ATBF1 (2107-2147 aa.) corresponding to the identical amino acid sequence of mouse ATBF1 (2114-2154 aa.)
Reactivity [Gene ID]

Human[463], Mouse[11906], Rat[307829]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names ZFHX3, ATBT, ZNF927
Background ATBF1 (also known as ZFHX3) is a transcription factor that has two-protein isoforms, the 404 kDa ATBF1-A and the 306 kDa ATBF1-B. ATBF1-A contains four homeodomains and 23 zinc-finger motifs.
ATBF1-B contains four homeodomains and 18 zinc fingers. ATBF1 is identified as DNA-binding protein, which binds to an AT-rich element of the human α-fetoprotein (AFP) gene, as a result suppressing its transcription activity. ATBF1 is also involved in cell cycle arrest and cooperating with p53 to activate the p21Waf1/Cip1 promoter. ATBF1 is expressed in the differentiation fields in association with β-tubulin III and MAP2 that are the neuronal differentiation marker. ATBF1 plays a crucial role in neuronal development and cell cycle arrest.
Related products PD011 Anti-ATBF1 (AT-6) pAb
Product category Research area:Cancer
Data





Citations

Western Blotting

  1. Zhang S et al. AT motif binding factor 1 (ATBF1) is highly phosphorylated in embryonic brain and protected from cleavage by calpain-1. Biochem Biophys Res Commun. 427, 537-41 (2012)(PMID:23022192)
  2. Chen XH et al. Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis. Acta Pharmacol Sin. 40, 1568-1577 (2019)(PMID:31152485)
  3. Zhang Z et al. Zfhx3 is required for the differentiation of late born D1-type medium spiny neurons. Exp Neurol. 322, 113055 (2019)(PMID:31491374)

Immunocytochemistry

  1. Kawaguchi M et al. A diagnostic marker for superficial urothelial bladder carcinoma: lack of nuclear ATBF1 (ZFHX3) by immunohistochemistry suggests malignant progression. BMC Cancer 16, 805 (2016)(PMID:27756245)

Immunohistochemistry

  1. Kim TS et al. The ZFHX3 (ATBF1) transcription factor induces PDGFRB, which activates ATM in the cytoplasm to protect cerebellar neurons from oxidative stress. Dis Model Mech. 3, 752-62 (2010)(PMID:20876357)
  2. Uhm KO et al. ATBF1 is a novel amyloid-β protein precursor (AβPP) binding protein that affects AβPP expression. J Alzheimers Dis. 43, 243-57 (2015)(PMID:25079792)
  3. Zhang Z et al. Zfhx3 is required for the differentiation of late born D1-type medium spiny neurons. Exp Neurol. 322, 113055 (2019)(PMID:31491374)
  4. Dong G et al. ZFHX3 Promotes the Proliferation and Tumor Growth of ER-Positive Breast Cancer Cells Likely by Enhancing Stem-Like Features and MYC and TBX3 Transcription. Cancers (Basel). 12, 3415 (2020)(PMID:33217982)

Immunofluorescence

  1. Jung CG et al. Homeotic factor ATBF1 induces the cell cycle arrest associated with neuronal differentiation. Development 132, 5137-45 (2005)(PMID:16251211)
  • The availability is based on the information in Japan at 17:00, Apr 23, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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