Code No. |
PD009 |
Anti-rck (p54) pAb
|
Price |
¥57,200
|
Size |
100 µL |
Availability (in Japan) |
10 or more
(In Japan at 00:05, Apr 24, 2024 in JST)
|
Clonality |
Polyclonal |
Clone |
Polyclonal |
Isotype
(Immunized Animal) |
Rabbit Ig (aff.) |
Applications |
WB 1:1,000 IC* reported. (PMID: 22674998 / 21109527 / 19812249) IH 1:100
|
Immunogen
(Antigen) |
synthetic peptide KLH-STARTENPVIC |
Reactivity [Gene ID] |
Human[1656], Mouse[13209], Rat[500988], Monkey*(PMID: 19458173) |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2 |
Storage temp. |
-20°C |
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
DDX6, HLR2 |
Background |
The lymphoma-associated rck (p54) gene product is a member of the DEAD box protein/RNA helicase family which has a variety of functions such as translation initiation, pre-mRNA splicing and ribosome assembly. Overexpression of human rck in a guinea pig cell line strongly inhibited the cell growth, and rck (p54) overexpression was found in >65% of colorectal tumours and >90% of hepatocellular carcinomas. Expression in tumors highly correlates with c-myc expression (>92%), suggesting rck (p54) may contribute to cell proliferation and carcinogenesis by increasing synthesis of c-myc protein via increased translation initiation of c-myc mRNA. |
Related products |
RN033P Anti-TNRC6A (GW182) (Human) pAb RN003M Anti-EIF2C2 (AGO2) (Human) mAb
|
Product category |
Research area:RNA-RNP Network Cancer |
Data |
|
Citations |
Western Blotting - Llorens F et al. A highly expressed miR-101 isomiR is a functional silencing small RNA. BMC Genomics. 14, 104 (2013)(PMID:23414127)
- Pérez-Vilaró G et al. Hepatitis C virus infection alters P-body composition but is independent of P-body granules. J Virol. 86, 8740-9 (2012)(PMID:22674998)
- Melemedjian OK et al. Targeting adenosine monophosphate-activated protein kinase (AMPK) in preclinical models reveals a potential mechanism for the treatment of neuropathic pain. Mol. Pain 7, 70 (2011)(PMID:21936900)
- Yao B et al. Divergent GW182 functional domains in the regulation of translational silencing. Nucleic Acid Res. 39, 2534-47 (2011)(PMID:21131274)
- Broytman O et al. Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines. Neurobiol. Aging 30, 1962-74 (2009)(PMID:18378046)
- Pauley KM et al. Formation of GW/P bodies as marker for microRNA-mediated regulation of innate immune signaling in THP-1 cells. Immunol Cell Biol. 88, 205-12 (2010)(PMID:19918258)
- Lian S et al. Small interfering RNA-mediated silencing induces target-dependent assembly of GW/P bodies. Mol Cell Biol. 18, 3375-87 (2007)(PMID:17596515)
Immunoprecipitation - Yu SF et al. The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome. PLoS Pathog. 7, e1002303 (2011)(PMID:22022269)
Immunocytochemistry - Pérez-Vilaró G et al. Hepatitis C virus infection alters P-body composition but is independent of P-body granules. J Virol. 86, 8740-9 (2012)(PMID:22674998)
- Swetloff A et al. Dcp1-bodies in mouse oocytes. Mol Biol Cell. 20, 4951-61 (2009)(PMID:19812249)
- Carlos TS et al. Parainfluenza virus 5 genomes are located in viral cytoplasmic bodies whilst the virus dismantles the interferon-induced antiviral state of cells. J Gen Virol. 90, 2147-56 (2009)(PMID:19458173)
- Zeitelhofer M et al. Dynamic interaction between P-bodies and transport ribonucleoprotein particles in dendrites of mature hippocampal neurons. J Neurosci. 28, 7555-62 (2008)(PMID:18650333)
- Kino Y et al. Intracellular localization and splicing regulation of FUS/TLS are variably affected by amyotrophic lateral sclerosis-linked mutations. Nucleic Acid Res. 39, 2781-98 (2011)(PMID:21109527)
- Moon SL, Parker R. Analysis of eIF2B bodies and their relationships with stress granules and P-bodies. Sci Rep. 8, 12264 (2018)(PMID:30115954)
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