Antibody detailed product information

Code No. MS-20-3
Anti-Ash (Grb2) mAb
Price ¥52,800
Size 100 µL (1 mg/mL)
Availability (in Japan) 1-9
（In Japan at 17:00, Apr 23, 2024 in JST）
Clonality Monoclonal
Clone 3F2
Isotype
(Immunized Animal) 		Mouse IgG1 κ
Applications WB　2-10 µg/mL  
IP*　reported.  
IF　reported.  （PMID: 17202467）
Immunogen
(Antigen)		 Recombinant Ash/Grb-2
Reactivity [Gene ID]

Human[2885], Mouse[14784], Rat[81504]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names growth factor receptor bound protein 2, Grb3-3, MST084, NCKAP2, MSTP084, EGFRBP-GRB2
Background Grb2 (Growth factor receptor bound protein 2) also known as Ash, is a 25-28 kDa molecule composed of central Src homology (SH2) domain and SH3 domains. Grb2 associates with tyrosine phosphorylated proteins such as EGFR, PDGFR, IRS-1, Shc and Gab1 via its Sh2 domain. The SH3 domain of Grb2 binds to Sos, which stimulates the GTP binding activity of Ras, cause activation of MAP kinase and other signaling pathways. Grb2 is an adaptor protein, which does not have enzymatic activity itself. Grb2 express ubiquitously and has two isoforms.
Product category Research area:Signal transduction
Data

Citations

Western Blotting

  1. Odai H et al. The proto-oncogene product c-Cbl becomes tyrosine phosphorylated by stimulation with GM-CSF or Epo and constitutively binds to the SH3 domain of Grb2/Ash in human hematopoietic cells.  J Biol Chem. 270, 10800-5 (1995)(PMID:7537740)
  2. Wada H et al. Establishment and molecular characterization of a novel leukemic cell line with Philadelphia chromosome expressing p230 BCR/ABL fusion protein. Cancer Res. 55, 3192-6 (1995)(PMID:7650032)
  3. Ueno H et al. An epidermal growth factor receptor-leukocyte tyrosine kinase chimeric receptor generates ligand-dependent growth signals through the Ras signaling pathway. J Biol Chem. 270, 20135-42 (1995)(PMID:7650032)
  4. Ueno H et al. Growth and survival signals transmitted via two distinct NPXY motifs within leukocyte tyrosine kinase, an insulin receptor-related tyrosine kinase. J Biol Chem. 271, 27707-14 (1996)(PMID:8910363)
  5. Ueno H et al. Antisense repression of proto-oncogene c-Cbl enhances activation of the JAK-STAT pathway but not the ras pathway in epidermal growth factor receptor signaling. J Biol Chem. 272, 8739-43 (1997)(PMID:9079708)
  6. Odai H et al. Purification and molecular cloning of SH2- and SH3-containing inositol polyphosphate-5-phosphatase, which is involved in the signaling pathway of granulocyte-macrophage colony-stimulating factor, erythropoietin, and Bcr-Abl. Blood 89, 2745-56 (1997)(PMID:9108392)

Immunoprecipitation

  1. Wada H et al. Establishment and molecular characterization of a novel leukemic cell line with Philadelphia chromosome expressing p230 BCR/ABL fusion protein. Cancer Res. 55, 3192-6 (1995)(PMID:7650032)

Immunofluorescence

  1. Odai H et al. The proto-oncogene product c-Cbl becomes tyrosine phosphorylated by stimulation with GM-CSF or Epo and constitutively binds to the SH3 domain of Grb2/Ash in human hematopoietic cells.  J Biol Chem. 270, 10800-5 (1995)(PMID:7537740)
  2. Shinoda T et al., DISC1 regulates neurotrophin-induced axon elongation via interaction with Grb2. J Neurosci. 27, 4-14. 2007(PMID:17202467)
  • The availability is based on the information in Japan at 17:00, Apr 23, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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