Antibody detailed product information

Code No.	MK-12-3
Anti-Ubiquitin mAb
Price	¥61,600
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 17:00, Apr 18, 2024 in JST）
Clonality	Monoclonal
Clone	2C5
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB　5 µg/mL IP*　reported. （PMID: 10488153） IC*　reported. （PMID: 10906068）
Immunogen (Antigen)	Bovine erythrocyte ubiquitin
Reactivity [Gene ID]	Human, Mouse, Rat, Bovine
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Background	Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. There are several reports showed that intracellular abnormal and short-lived proteins are degraded through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
Related products	MK-11-3 Anti-Ubiquitin mAb D058-3 Anti-Multi Ubiquitin mAb D058-8 Anti-Multi Ubiquitin mAb-Agarose D071-3 Anti-Multi Ubiquitin mAb M113-3 Anti-SUMO-1 mAb M114-3 Anti-SUMO-2/3 mAb M162-3 Anti-p62 (SQSTM1) (Human) mAb PM045 Anti-p62 (SQSTM1) pAb
Product category	Research area:Ubiquitin proteasome
Data
Citations	Western Blotting Sutovsky P et al. Ubiquitinated sperm mitochondria, selective proteolysis, and the regulation of mitochondrial inheritance in mammalian embryos. Biol Reprod. 63, 582-90 (2000)(PMID:10906068) Ott DE et al. Retroviruses have differing requirements for proteasome function in the budding process. J Virol. 77, 3384-93 (2003)(PMID:12610113) Immunoprecipitation Hiyama H et al. Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome. J Biol Chem. 274, 28019-25 (1999)(PMID:10488153) Immunocytochemistry Sutovsky P et al. Ubiquitinated sperm mitochondria, selective proteolysis, and the regulation of mitochondrial inheritance in mammalian embryos. Biol Reprod. 63, 582-90 (2000)(PMID:10906068)

The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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